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| HPLC法测定小儿热速清糖浆中葛根素的含量 | |
| 引用本文： | 李延雪,王云龙,姜雪敏. HPLC法测定小儿热速清糖浆中葛根素的含量[J]. 中国药师, 2012, 15(6): 830-832 |
| 作者姓名： | 李延雪 王云龙 姜雪敏 |
| 作者单位： | 鸡西市食品药品检验检测中心,黑龙江鸡西,158100 |
| 摘 要： | 目的:建立HPLC法测定小儿热速清糖浆中葛根素的含量.方法:采用Agilent Extend-C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水(11:89)为流动相,流速1.0 ml·min-1,检测波长250 nm,柱温25℃.结果:葛根素在0.018 5～0.370 8 μg范围内与峰面积呈良好的线性关系(r=1.000 0),平均回收率为98.21%,RSD为0.79%(n=6).结论:本方法简便、准确,重复性好,可用于小儿热速清糖浆中葛根素的测定. |
| 关 键 词： | 葛根素 小儿热速清糖浆 高效液相色谱法 |
| 收稿时间： | 2012-01-01 |
| 修稿时间： | 2012-02-28 |
Determination of Puerarin in Xiaoer Resuqing Syrups by HPLC |
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| Li Yanxue,Wang Yunlong and Jiang Xuemin. Determination of Puerarin in Xiaoer Resuqing Syrups by HPLC[J]. China Pharmacist, 2012, 15(6): 830-832 | |
| Authors: | Li Yanxue Wang Yunlong Jiang Xuemin |
| Affiliation: | (Institute for Food and Drug Control of Jixi City,Heilongjiang Jixi 158100,China) |
| Abstract: | Objective: To establish an HPLC method for the determination of puerarin in Xiaoer Resuqing syrups.Method: An Agilent Extend-C18(250 mm×4.6 mm,5 μm) column was used with acetonitrile-water(11∶ 89) as the mobile phase at a flow rate of 1.0 ml·min-1.The detection wavelength was 250 nm and the column temperature was 25℃.Result: Puerarin showed good linearity within the range of 0.018 5-0.370 8 μg(r=1.000 0).The average recovery was 98.21% with RSD of 0.79%(n=6).Conclusion: The method is simple,accurate and reproducible for the determination of puerarin in Xiaoer Resuqing syrups. |
| Keywords: | Puerarin Xiaoer Resuqing syrups HPLC |
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