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| 苦参碱对人突变肉瘤细胞系MS0812的抑制增殖和诱导凋亡体外实验 | |
| 引用本文： | 张松,杨立业,李雪,姜翠玉.苦参碱对人突变肉瘤细胞系MS0812的抑制增殖和诱导凋亡体外实验[J].中国药师,2012,15(6):764-766. |
| 作者姓名： | 张松 杨立业 李雪 姜翠玉 |
| 作者单位： | 1. 汕头大学医学院,广东汕头,515041;中山大学附属东华医院 2. 汕头大学医学院,广东汕头,515041;广东潮州市中心医院 3. 汕头大学医学院,广东汕头,515041 4. 中山大学附属东华医院 |
| 基金项目： | 国家自然科学基金项目(编号：30672359) |
| 摘 要： | 目的:观察苦参碱(matrine)对体外培养的人肉瘤细胞系MS0812细胞的增殖和诱导调亡作用.方法:苦参碱处理人肉瘤细胞系MS0812后,应用MTT比色法检测不同浓度苦参碱和不同作用时间对MS0812细胞的增殖作用的影响,TUNEL法和流式细胞仪检测细胞凋亡.结果:苦参碱浓度大于500.0 mg·L-1作用24h对MS0812 细胞增殖有抑制作用;通过TUNEL试剂盒可以发现凋亡阳性细胞;流式细胞仪检测随着苦参碱浓度的增加,凋亡率逐渐升高.结论:苦生碱体外对肿瘤细胞系MS0812的生长抑制和诱导凋亡作用有限. |
| 关 键 词： | 苦参碱 凋亡 肉瘤 |
| 收稿时间： | 2011/12/11 0:00:00 |
| 修稿时间： | 2012/3/20 0:00:00 |
Effects of Matrine on Proliferation and Apoptosis of Human Mutated Sarcoma Cell Line MS0812 |
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| Zhang Song , Yang Liye , Li Xue , Jiang Cuiyu.Effects of Matrine on Proliferation and Apoptosis of Human Mutated Sarcoma Cell Line MS0812[J].China Pharmacist,2012,15(6):764-766. | |
| Authors: | Zhang Song Yang Liye Li Xue Jiang Cuiyu |
| Institution: | 1.The Medical School of Shantou University,Guangdong Shantou 515041,China;2.Chaozhou Hospital of Guangdong;3.Donghua Hospital of Zhongshan University) |
| Abstract: | Objective: To observe the effects of matrine on the proliferation and apoptosis of human mutated sarcoma cell line MS0812.Method: Human sarcoma cell line MS0812 were treated with matrine in different concentration and time.The inhibitory rates were detected by MTT assay.Flow cytometry and TUNEL method were utilized to observe and detect the apoptosis of MS0812 cells induced by matrine.Result: Matrine above 500.0 mg·L-1 exhibited significant inhibitory effect on the growth of human cancer cell line MS0812.TUNEL-kit found apoptosis positive cells,and the apoptosis rate was increased with the matrine concentration increase analyzed by flow cytometry.Conclusion: Matrine shows the limited effects on the apoptosis and inhibition of human sarcoma cell line MS0812 in vitro. |
| Keywords: | Matrine Apoptosis Sarcoma |
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