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| 淋球菌耐青霉素质粒转化大肠杆菌的研究 | |
| 引用本文： | 冯国和 陈庆学. 淋球菌耐青霉素质粒转化大肠杆菌的研究[J]. 中国医科大学学报, 1994, 23(6): 545-548 |
| 作者姓名： | 冯国和 陈庆学 |
| 作者单位： | Dept.of Infectious Disease，The Second Affiliated Hospital |
| 摘 要： | 应用碱裂解法从淋球菌Ｅ参考菌株中获得约７．２ｋｂ耐青霉素质粒ＤＮＡ，并转化大肠杆菌ＨＢ１０１内。采用ＤＮＡ直接酶标——化学发光自显影技术，标记４．３ｋｂｐＢＲ３２２质粒为探针，通过Ｓｏｕｔｈｅｒｎ印迹杂交加以鉴定，本实验所获含耐青霉素质粒的大肠杆菌ＨＢ１０１，将为淋球菌耐青霉素质粒探针的制备，检测耐青霉素基因打下基础。 |
| 关 键 词： | 淋球菌；耐青霉素质粒；转化；化学发光自显影 |
The Transformation of Penicillin-Resistant Plasmids in Neisseria Gonorrhoeae to Escherichia Coli |
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| Feng Guohe,Chen Qingxue,Qiao Guangyan,et al. The Transformation of Penicillin-Resistant Plasmids in Neisseria Gonorrhoeae to Escherichia Coli[J]. Journal of China Medical University, 1994, 23(6): 545-548 | |
| Authors: | Feng Guohe Chen Qingxue Qiao Guangyan et al |
| Abstract: | About 7.2kb penicillin-resistant plasmid DNA was obtained from the reference strain-E of Neisse-ria gonorrhoeae,and was transfered to strain-HB101 of Escherichia Coli.Direct DNA labelling-chemolu-minescence photographic detection was used to label 4.3kb pBR322 plasmid probe.Transformation wasidentified by southern blotting hybridization,Strain-HB101 of Escherichia Coli obtained in the test whichcontained about 7.2kb penicillin-resistant plasmid DNA could be used to prepare the probe of penicillin-resistant plasmid of neisseria gonorrhoeae. The foundation was established for the exannnatjon of peni-cillin-resistant gene of Neisseria gonorrhoeae |
| Keywords: | neisseria gonorrhoese penicillin-resistant plasmid transformation chemolumi-nescence photography |
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