大鼠胎鼠成骨细胞的培养及初步鉴定

目的：获取较纯的大鼠（SD）胎鼠成骨细胞，为生物学评价奠定基础。方法：从SD临产孕鼠腹中取出胎鼠，机械剥离其颅骨片，酶消化法与组织块法结合培养原代混合细胞，采用差速贴壁法对成骨细胞进行纯化，不同染色法进行鉴定。结果：原代培养的细胞为混杂细胞，经2～3次纯化可完全去除纤维样细胞。成骨细胞呈三角形、多边形，碱性磷酸酶和钙结节染色阳性。结论：实验获取较纯的大鼠胎鼠成骨细胞，符合体外细胞生物学评价试验中对细胞的要求。

The cultivation and preliminary identification of osteoblast from rat fetal

Objective:To obtain pure fetal rat osteoblast from rats. Method:SD parturient pregnant fetal rat's ab-domen,mechanical spin off its skull,enzyme digestion method and tissue block method combined with cultivating the origi-nal generation of hybrid cells,using the differential sticking wall method to purification of osteoblast,different staining method for identification. Result:the primary cultured cells,after two or three sample purification can completely remove fiber cells. Osteoblast is triangular,polygons,alkaline phosphatase and calcium nodules staining positive. Conclusion:the experiment to obtain relatively pure fetal rat osteoblast in rats,conform to the evaluation of in vitro cell biology experiment requirement to the cells.