Genetic analysis of sexual hybrids and protoplast fusion products of the yeast Saccharomycopsis lipolytica

Protoplast fusion of genetically marked Saccharomycopsis lipolytica strains was carried out succesfully. Mitotic segregation was induced to prove the presence of parental markers in fusion products. Methylbenzimidazol-2-yl-carbamate has been shown to induce efficiently mitotic segregation in this yeast. The segregation pattern of genetic markers in a sexual hybrid was compared to that of a parasexual hybrid.