树脂充填诱发猫牙髓神经反应的免疫组织化学研究

目的观察牙髓神经对树脂充填深窝洞后疼痛性反应的变化规律。方法应用免疫组织化学染色的方法,对树脂充填深龋洞后不同时期P物质表达变化进行观察研究。结果在树脂充填侧的成牙本质细胞及胞质突区域,充填后第1、3、5天与未充填对照组相比,P物质染色均呈明显阳性显色(P<0.05),第7天充填组与对照组显色程度接近(P>0.05)。结论树脂直接充填深窝洞刺激牙髓神经产生的疼痛反应在第1~3天达到高峰,随后逐渐减弱。

Immunohistochemistry Researches of Pulpal Nerve Pain Response to Stimulation of Direct Resin Composite Filling

Objective To evaluate the long term pulpal pain response in terms of time-sequenced changes to the stimulation of composite filling. Methods Immunohistochemistry method was used to stain the substance P(SP) in pulpal tissue which could directly reflect the activation of nerve by stimuli. Results In the area of odontoblast and their protrusions, the SP-immunoreactive nerve fibers were stained, the grayscales of the groups which on day 1, 3, 5 after composite filling was significantly higher than control group (P<0.05), and there was no significant difference between control group and the experimental group on day 12 after composite filling(P>0.05). Conclusion The results show that the pain response induced by deep cavity composite filling may last for several days with peak response on day 1 to 3 and may alleviate as time pass-by.