| 维生素D缺乏动物模型的建立 |
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| 引用本文： | 常正奇,张泽华,罗飞,许建中. 维生素D缺乏动物模型的建立[J]. 重庆医学, 2011, 40(11): 1041-1043. DOI: 10.3969/j.issn.1671-8348.2011.11.001 |
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| 作者姓名： | 常正奇 张泽华 罗飞 许建中 |
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| 作者单位： | 常正奇,Chang Zhengqi(山东潍坊解放军第八十九医院);张泽华,罗飞,许建中,Zhang Zehua,Luo Fei,Xu Jianzhong(第三军医大学西南医院骨科/全军矫形外科中心,重庆,400038) |
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| 基金项目： | 国家自然科学基金青年基金 |
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| 摘 要： | 目的采用BALB/c小鼠建立维生素D(VD)缺乏模型并进行评价。方法将BALB/c小鼠分成正常组、VD+组及VD-组,测4~10、12周小鼠体质量,并观察其运动习性,8、12周期间饮食消耗;12周时测血浆VD、清蛋白水平;将VD缺乏老鼠10只分为组Ⅰ继续遮光加正常饮食,组Ⅱ正常环境加正常饮食,6周后测VD水平。结果正常组与VD+组体质量比较差异无统计学意义(P>0.05);VD+组与VD-组8周时日平均消耗饲料,有差异,12周时无差异;正常组、VD+组、VD-组血浆清蛋白分别为(33.2±1.04)、(32.4±0.53)、(32.8±0.67)g/L,差异无统计学意义;正常组与VD+组血浆VD水平无显著差异,VD-组血浆VD水平下降到正常的20%以下;6周后,组Ⅰ、组ⅡVD水平分别为(18.46±1.53)、(58.46±5.53)nmol/L(P<0.001)。结论通过控制维生素来源建立维生素D缺乏BALB/c小鼠动物模型是可行的。
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| 关 键 词： | 维生素D缺乏 维生素D生理作用 BALB/c小鼠 动物模型 |
To establish the animal model of vitamin D deficiency |
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| Chang Zhengqi,Zhang Zehua,Luo Fei,Xu Jianzhong. To establish the animal model of vitamin D deficiency[J]. Chongqing Medical Journal, 2011, 40(11): 1041-1043. DOI: 10.3969/j.issn.1671-8348.2011.11.001 |
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| Authors: | Chang Zhengqi Zhang Zehua Luo Fei Xu Jianzhong |
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| Affiliation: | Chang Zhengqi#,Zhang Zehua,Luo Fei,Xu Jianzhong(Department of Orthopaedics,Southwest Hospital,Third Military Medical University,Chongqing 400038 China) |
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| Abstract: | Objective To establish and evaluate the animal model of vitamin d deficiency using BALB/c mice.Methods Put BALB/c mice into normal group,VD+ group andVD-group,measured the weight of the three groups of 4-10,12th week;observed the mouse activity;Recorded food consumption during 8 and 12th week;Assay for serum albumin and 25-hydroxyvitamin D level after 12 weeks.Then 10 mouse of vitamin D deficiency were divided into two groups with normal diet for six weeks,but one group as beginning the other one as normal ... |
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| Keywords: | vitamin D deficiency physiological effects of vitamin D BALB/c mice animal model |
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