组蛋白去乙酰化酶1在胰腺癌表达及临床意义

目的 探讨胰腺癌组蛋白去乙酰化酶1(histone deacetylase1,HDAC1)表达的临床意义.方法 收集30例胰腺癌和相应癌旁组织,应用实时定量PCR和免疫组织化学方法检测HDAC1的表达,并分析胰腺癌HDAC1表达与临床病理参数的关系.结果 胰腺癌HDAC1 mRNA表达指数为2.60(0.42～12.81),癌旁组织为1.02(0.19～3.58),两组表达有显著差异(P=0.001).胰腺癌HDAC1蛋白表达阳性细胞率为(56±26)%,癌旁组织为(6±6)%,相差显著(P=0.000).以阳性细胞率平均值56%为界,高表达(≥56%)18例,低表达(＜56%)12例.胰腺癌HDAC1高表达和肿瘤TNM分期、淋巴结转移相关.结论 胰腺癌HDAC1高表达提示肿瘤可能已属晚期,预后不良.

HDAC1 expression in pancreatic adenocarcinoma and it clinic significance

Objective To investigate the clinic significance of HDAC1 expression in human pancreatic carcinoma.Methods 30 samples of pancreatic carcer tissues and paracancerous tissues were collected.HDAC1 expression was detected by real-time PCR and immunohistochemistry techniques.The relationships between clinicopathological findings and the expression of HDAC1 were analyzed. Results The RQ level of HDAC1 mRNA expression in human pancreatic carcinoma tissues and paracancerous tissues was 2.60(0.42～12.81)and 1.02(0.19～3.58),respectively(P=0.001).The percentage of positive cells expressed HDAC1 protein in human pancreatic carcinoma tissues and paracancerous tissues were(56 ±26)%and(6±6)%,respectively(P=0.000).The highly expressed HDAC1 correlated significantly with an advanced TNM stage and lymph node metastasis.Conclusions In pancreatic carcinoma tissues,highly expressed HDAC1may indicate the status of advanced TNM stage and poor prognosis.