羟基积雪草苷在大鼠肠内吸收的研究

目的 研究羟基积雪草苷在大鼠肠段各段的吸收特征，探究其在肠道的吸收机制，为设计药物的剂型和给药途径提供理论基础。方法 利用大鼠在体循环肠灌流模型和方法，探明羟基积雪草苷的肠道吸收部位和吸收特征，以及不同表面活性剂对其肠吸收的影响。结果/b> 羟基积雪草苷在大鼠小肠段吸收强于大肠段，其吸收速率回肠＞空肠≥十二指肠＞结肠。而不同浓度的羟基积雪草苷溶液回肠吸收率和吸收速率常数无显著差异。在3种不同表面活性剂中，聚山梨酯-80对羟基积雪草苷的回肠吸收具有显著的促进作用。结论 羟基积雪草苷的肠吸收主要集中在小肠中段，且吸收机制推测为被动扩散，表面活性剂聚山梨酯-80可以促进其回肠吸收。

Absorption mechanism of madecassoside in intestinal of rats Analysis on DNA damage of Chinese hamster lung fibroblas cell line

Objective To investigate the absorptive characteristics of madecassoside in different intestinal segments of rats, to observe its intestinal absorption mechanism, and to provide rational foundation for its dosage form and administration route design. Methods In situ intestinal perfusion model was used to suudy the best absorptive site, absorptive characteristics, and transport behavior influenced by three different surfactants. Results The results showed that the absorption of madecassoside in small intestine was much higher than that in large intestine, and the rank order of intestinal permeability was ileum > jejunum > duodenum > colon; There was no significant difference of absorption parameters of madecassoside at various concentrations; Among three surfactanrs, Tween-80 could signifantly improve the intestinal absorption of madecassoside. Conclusion Main absorptive segments are middle site of small intestine, transport mechanism is similar to passive diffusion, and Tween-80 could enhance madecassoside absorption in ileum.