SCID小鼠体内人体肿瘤移植及其人体免疫功能重建

目的观察高转移性人肺巨细胞癌系（ＰＧ）及转染了白细胞介素６基因的ＰＧ细胞（ＰＧＴＳ７）在ＳＣＩＤ小鼠及免疫重建ＳＣＩＤ小鼠体内的生长及转移行为,阐明ＰＧＴＳ７自分泌的白细胞介素６增强人体免疫的抗肿瘤作用。方法皮下移植人体肿瘤,部分受体鼠同时经腹腔注射人体外周血淋巴细胞。观察成瘤潜伏期、成瘤率、生长速度、体积、肺及淋巴结转移率和小鼠血清中人体免疫球蛋白（ＨＩｇ）的含量。结果全部动物见肿瘤生长,但ＰＧＴＳ７在免疫重建鼠体内成瘤潜伏期延长,肿瘤体积小、淋巴结转移率低,而血清中ＨＩｇ含量高。结论（１）ＳＣＩＤ小鼠亦为研究ＰＧ及ＰＧＴＳ７生长及转移的良好动物。（２）ＰＧＴＳ７自分泌白细胞介素６能刺激小鼠体内人淋巴细胞增殖、活化,增加ＨＩｇ的产生,降低肿瘤生长及淋巴结转移。

Human tumor xenografted into SCID mice and human immune function reconstitution

Objective In order to observe (1) The behavior of growth and metastasis of PG and PGPTS7 in SCID mice and human immune function reconstituted mice; (2) The ability of interlukin 6 autosecreted from PGTS7 in enhancing the anti tumor activity of the peripheral blood lymphocytes (PBL). Methods Xenografting of PG and PGTS7 into the subepithelial space of the SCID mice, and in some of these animals, human PBL were administrated simultaneously into the peritoneal cavities. The latent period, taken rate, growth speed, volume of the grafted tumor, incidences of metastasis in lungs and lymph nodes and serum level of human immunoglobulin of the immunity reconstitued mice were examined. Results Although tumor growth had been detected in all the experimental animals, the latent period of grafted PGTS7 was postponed and the volume of tumor mass as well as the incidence of lymph node metastasis all became lower in the immunity reconstituted mice accompanied simultaneously with a higher serum level of human immunoglobulin (H Ig ). Conclusions (1) The SCID mice are good as an appropriated hosts in studying the hebavior of growth and metastasis of PG and PGTS7. (2) IL 6 autosecreted from PGTS7 stimulates the proliferation and promotes the activating of PBL. It seems also able to enhance the liberation of human immunoglobulin: to supress the growth of tumor cells and to reduced the rate of lymph node metastasis.