Increased in vitro lipid peroxidation of gerbil cerebral cortex as compared with rat |
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| Authors: | J A DeLeo R A Floyd J M Carney |
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| Affiliation: | 1. Department of Pharmacology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190 U.S.A.;2. Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104 U.S.A.;1. Key Lab of the Basic Pharmacology of the Ministry of Education, Zunyi Medical University, Zunyi, 563006, China;2. Zunyi Tobacco Monopoly Bureau, Zunyi, 563000, China;3. Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China;4. College of Pharmacy, Zunyi Medical University, Zunyi, 563006, China;5. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia;1. Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic;2. Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic;3. Laboratory of Molecular Modeling Applied to the Chemical and Biological Defense (LMCBD), Military Institute of Engineering, Rio de Janeiro/RJ, Brazil;4. Department of Neurology, University Hospital Hradec Kralove, Hradec Kralove, 500 05, Czech Republic;5. College of Life Science, Yangtze University, Jingzhou, Hubei, China;6. School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;1. University of Hradec Kralove, Faculty of Science, Department of Chemistry, Rokitanskeho 62, 500 03 Hradec Kralove, Czech Republic;2. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic;3. Department of Experimental Biology, Faculty of Science, Masaryk University, CZ-625 00 Brno, Czech Republic;4. University Hospital in Hradec Kralove, Biomedical Research Centre, Sokolska 581, 500 05 Hradec Kralove, Czech Republic |
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| Abstract: | ![]()
The in vitro thiobarbituric acid test was used as a measure of lipid peroxidation in the gerbil and rat. Synaptosomal preparations were isolated from the cerebral cortex of each species and incubated with a free radical generating system. Varying concentrations of ADP-Fe3+, with ascorbate and oxygenated incubation medium were used to generate hydroxy-radicals. Peroxidation of the synaptosomal membrane lipids was determined using malondialdehyde (MDA) accumulation. Both the gerbil and rat demonstrated significant increases in MDA in the presence of the generating system, while the gerbil P2 fraction consistently showed an increased level of MDA accumulation as compared with rat at each of the concentrations of ADP-Fe3+. Across a range of concentrations, there was a 2-2.6-fold greater increase in MDA accumulation in gerbil as compared with rat. Free radical generation is currently thought to be involved in the associated damage following cerebral ischemia. An in vitro model capable of producing biochemically similar damage to membrane systems by means of a controlled free-radical generating system may prove useful in studying possible mechanisms of ischemic damage. |
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