| 川芎嗪联合环胞霉素A逆转白血病多药耐药的研究 |
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| 引用本文： | 杨岚,杨平地,梁蓉. 川芎嗪联合环胞霉素A逆转白血病多药耐药的研究[J]. 癌症, 2000, 19(4): 304-306 |
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| 作者姓名： | 杨岚 杨平地 梁蓉 |
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| 作者单位： | [1]第四军医大学西京医院血液科 [2]海军总医院血液科 |
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| 摘 要： | 目的：寻找有效的多药耐药联合逆转剂。方法：用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）,Ｗｅｓｔｅｒｎ ｂｌｏｔ和ＭＴＴ法研究芎嗪逆转耐药细胞系ＨＬ－６０／ＶＥＲ的作用,对多种化疗药物耐药的白血病用川芎嗪与环孢菌素Ａ（ＣｓＡ）加化疗药物联合用于治疗２１例复发难治性白血病。结果：单用川芎嗪在体外能不同程度地增加ＨＬ－６０／ＶＣＲ细胞对多种化疗药物的敏感性,降低Ｐ１７０蛋白的表达。川芎嗪联合不胞素Ａ有较好逆
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| 关 键 词： | 白血病 川芎嗪 多药耐药性 环孢霉素A RTPCR |
| 修稿时间： | : |
Study on reversal of multidrug resistance in leukemia by tetramethylphylpyrazine combining with cyclosporin A |
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| Abstract: | Objective: To search effective combination of multidrug resistance(MDR) modulators Methods:The reversal effects of tetramethylphylpyrazine(TMPZ)on the drug resistance of HL-60/VCR was evaluated by utilizing RT-PCR,Western blot and MTT Twenty one refractory and relapsed leukemia patients were received chemotherapy,combined with TMPZ and cyclosporin A(CsA) Results: Sensitivity of HL-60/VCR to multidrug chemotherapy was enhanced and p170 expression was decreased by the single application of TMPZ The reversal effectiveness of the TMPZ in combination with CsA to multidrug resistance of refractory and relapsed leukemia was favorable The complete remission(CR) rate was 44 4%and partial remission(PR) rate was 33 3%in the treatment group Conclusion: TMPZ in combination with CsA might be a safe and effective MDR reversing manipulation,and can be applied in the chemotherapy of refractory and relapsed acute leukemia |
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| Keywords: | Leukemia Tetramethylphylpyrazine Multidrug resistance |
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