| 重组人内皮抑素的初纯工艺探索 |
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| 引用本文： | 张兆林,吴芸,方悦群,梁军,徐根兴,肖祥华. 重组人内皮抑素的初纯工艺探索[J]. 南京军医学院学报, 2002, 24(2): 93-95 |
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| 作者姓名： | 张兆林 吴芸 方悦群 梁军 徐根兴 肖祥华 |
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| 作者单位： | 1. 苏州中凯生物药业有限公司,江苏,苏州,215128
2. 南京军医学院分子医学研究所,江苏,南京,210099 |
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| 摘 要： | 目的：通过对比实验，找出一条成本低、纯度高的重组人内皮抑素（rhEndostatin)纯化工艺路线。方法：将rhEndostatin包涵体裂解后用缓冲液稀释至不同尿素（Urea)浓度，上分子筛层析；再将含2－巯基乙醇（2－ME）和不含2－ME的样品分别上分子筛层析；将所得蛋白样品电泳，测定活性，比较结果。结果：使用不含Urea和2－ME的缓冲液所得样品纯度、活性、回收率均很好。结论：用不含Urea和不含2－ME的纯化方法是最佳工艺路线。
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| 关 键 词： | 重组人内皮抑素 纯化 分子层析法 |
| 文章编号： | 1009-1874(2002)02-0093-03 |
| 修稿时间： | 2002-01-21 |
Exploration of technology of primary purification in recombinant endostatin |
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| ZHANG Zhao-lin ,WU Yun ,FANG Yue-qun ,LIANG Jun ,XU Gen-xing ,XIAO Xiang-hua Zhongkai Biopharmaceutical Co. Ltd,Suzhou,Jiangsu ,China. Exploration of technology of primary purification in recombinant endostatin[J]. Journal of Nanjing Military Medical College, 2002, 24(2): 93-95 |
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| Authors: | ZHANG Zhao-lin WU Yun FANG Yue-qun LIANG Jun XU Gen-xing XIAO Xiang-hua Zhongkai Biopharmaceutical Co. Ltd Suzhou Jiangsu China |
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| Affiliation: | ZHANG Zhao-lin 1,WU Yun 1,FANG Yue-qun 1,LIANG Jun 2,XU Gen-xing 2,XIAO Xiang-hua 2 1Zhongkai Biopharmaceutical Co. Ltd,Suzhou,Jiangsu 215128,China, 2Institute of Molecular Medicine,Nanjing Military Medical College,Nanjing,Jiangsu 2100 |
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| Abstract: | Objective: To find a low-cost and high-purified technology in purifying recombinant endostatin (rhEndostatin) by using comparative experiments. Methods: The lysate of endostatin inclusion body was diluted with buffers to different concentrations (Urea), then it was chromatographied with molecular sieve: samples with or without 2-mercaptoethanol (2-ME) were chromatographied with molecular sieve. All the purified proteins were electrophoresied, their activities were measured and their results were compared. Results: The purity, activity and recovery rate of samples treated with or without Urea and 2-ME were satisfactory. Conclusion: The method of purification of rhEndostatin by using buffers without Urea and 2-ME is the best route. |
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| Keywords: | endostatin recombinant purification replication chromatography molecular sieve |
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