| 水中问号钩端螺旋体基因芯片检测 |
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| 引用本文： | 唐雨德,周东明,陈永红,郁兴明. 水中问号钩端螺旋体基因芯片检测[J]. 中国公共卫生, 2010, 26(5) |
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| 作者姓名： | 唐雨德 周东明 陈永红 郁兴明 |
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| 作者单位： | 南京军区疾病预防控制中心,南京,210002 |
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| 基金项目： | 南京军区卫生专业人才培养'122'工程资助项目 |
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| 摘 要： | 目的应用DNA微阵列芯片快速检测水中问号钩端螺旋体。方法应用基因芯片对不同浓度钩体水、钩体可能生存的不同水体、经水传播的其他病原及疫区可疑水标本进行检测,并以PCR技术为对照,观察基因芯片技术检测钩体的特异性、敏感性及可行性。结果用芯片和PCR技术对水中不同浓度钩体的检测结果表明,最低检测浓度分别为5和10条钩体/100 mL,芯片检测的敏感性高于常规PCR法;对钩体可能生存的不同生物与理化特性水体(非疫区)及经水传播的其他病原进行检测,结果均为阴性;对疫区20份可疑水标本进行检测,芯片、PCR与培养法检测结果阳性分别为8,5,4份。结论基因芯片技术可快速、灵敏、特异地检测水中问号钩端螺旋体。
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| 关 键 词： | 问号钩端螺旋体 基因芯片 水体 |
Detection of Leptospira interrogans in water with DNA chip |
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| TANG Yu-de,ZHOU Dong-ming,CHEN Yong-hong,et al.. Detection of Leptospira interrogans in water with DNA chip[J]. Chinese Journal of Public Health, 2010, 26(5) |
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| Authors: | TANG Yu-de ZHOU Dong-ming CHEN Yong-hong et al. |
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| Affiliation: | TANG Yu-de,ZHOU Dong-ming,CHEN Yong-hong,et al.Center for Disease Prevention , Control of Nanjing Military Comm,(Nanjing 210002,China) |
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| Abstract: | Objective To detect Leptospira interrogans in water with the DNA chip.Methods The water with different concentration of Leptospira interrogans and other pathogens were detected with DNA chip.The specificity and sensitivity of the DNA chip were observed with compaison of polymerase chain reaction(PCR).Results Positive signals were displayed on the microchips for water samples with Leptospira interrogans and there was no signal displayed for water samples without Leptospira interrogans.The least detection sen... |
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| Keywords: | Leptospira interrogans DNA chip water body |
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