Inhibition of adhesion of Clostridium difficile to human intestinal cells after treatment with serum and intestinal fluid isolated from mice immunized with nontoxigenic C. difficile membrane fraction |
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| Affiliation: | 1. MOA Key Laboratory of Crop Physiology, Ecology and Cultivation (The Middle Reaches of Yangtze River), Huazhong Agricultural University, Wuhan 430070, Hubei, China;2. Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064, Hubei, China;3. Soil Science Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, Kalyoubia, Egypt;1. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India;2. Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India;1. Aix-Marseille University, Unité de Recherche sur les Maladies Infectieuses Transmissibles et Emergentes, UMR 63, CNRS 7278, IRD 198, INSERM U1095, Marseille, France;2. Inserm UMR 1068, Centre de Recherche en Cancérologie de Marseille, Marseille, France;1. Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia;2. Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovakia;3. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1581 Budapest, Hungary;4. Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Athens 11527, Greece;1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;2. College of Life Sciences, Nankai University, Tianjin 300071, China;3. Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | ![]()
Diarrhea and pseudomembrane colitis caused by Clostridium difficile infection is a global health concern because of the high recurrence rate after standard antibiotic therapy. Vaccination presents a powerful countermeasure against disease recurrence. In this study, mice vaccinated with the nontoxigenic C. difficile membrane fraction generated a marked immune response to the antigen, as demonstrated by the serum IgG and intestinal fluid IgA levels. Significantly, pretreatment with harvested IgG- and IgA-containing fluids was sufficient to prevent in vitro adhesion of C. difficile to human Caco-2 intestinal cells. These results highlight the potential of nontoxigenic C. difficile membrane fraction as a vaccine candidate for C. difficile infection. |
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| Keywords: | Membrane fraction Adhesion Vaccine CDI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Clostridium difficile" },{" #name" :" __text__" ," _" :" infection ELISA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" enzyme-linked immunosorbent assay PCR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" polymerase chain reaction PBS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" phosphate-buffered saline PBST" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" PBS containing 0.05% (v/v) Tween 20 HRP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" horse radish peroxidase MOI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" multiplicity of infection ntCDMF" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" __text__" ," _" :" nontoxigenic " },{" #name" :" italic" ," _" :" C. difficile" },{" #name" :" __text__" ," _" :" membrane fraction |
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