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Genetic and antigenic characterization of H5N1 viruses of clade 2.3.2.1 isolated in India |
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| Affiliation: | 1. College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China;2. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China;3. Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease, Yangzhou, Jiangsu, PR China;4. Sinopharm Yangzhou Vac Biological Engineering Co. LtD, Yangzhou, Jiangsu 225009, PR China;5. The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou, Jiangsu 225009, PR China;1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou 510642, China;3. National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangzhou 510642, China;1. Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, China;2. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, China;3. Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225009, China |
| Abstract: | The recurrent circulation of highly pathogenic avian influenza (HPAI) H5N1 in Indian poultry since 2006 resulted in emergence of the viruses of distinct antigenic clades of haemagglutinin (HA) with the majority of the H5N1 outbreaks since 2011 belonging to clade 2.3.2.1. The present study was aimed to characterize the antigenic profile of a collection of H5N1 HPAI viruses of clade 2.3.2.1 isolated in India by applying antigenic cartography, serological data and phylogenetic analysis. Eleven H5N1 viruses (2 of clade 2.2 and 9 of clade 2.3.2.1) were selected based on genetic analysis and were further characterized by antigenic cartography analysis based on cross HI (hemagglutination inhibition) data. This study highlights the intercladal antigenic differences between clades 2.3.2.1 and 2.2 and the intracladal antigenic divergence among the clade 2.3.2.1 viruses. Five viruses of clade 2.3.2.1 were also studied for analysis of glycosylation pattern of Hemagglutinin (HA) gene and the growth kinetics analysis in MDCK cells in which the viruses CL03485/H5N1 and 03CL488/H5N1 showed better replication kinetics than other viruses. The study presents a baseline data of antigenicity and other factors that can be used in the selection of suitable H5 vaccine strains or HA donor viruses to develop H5 vaccine strains by reverse genetics or other methods for control of currently circulating H5N1 viruses in Indian region. |
| Keywords: | HPAI H5N1 Phylogenetic analysis Antigenic cartography Growth kinetics |
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