蛙皮素对PC-3细胞骨架及细胞内游离Ca~(2+)的影响

目的:观察蛙皮素(BBS)对前列腺癌PC-3细胞骨架形态及细胞内游离Ca2+(Ca2+]i)浓度的影响。方法:①利用免疫荧光法(IH),结合激光扫描共聚焦显微镜(LSCM)检测10-5mol/L浓度BBS处理的PC-3细胞角蛋白(CK)的表达,以反映其对细胞骨架形态的影响;②应用Fluo-3/AM荧光标记技术和LSCM检测不同浓度BBS(10-9、10-7、10-5mol/L)处理的PC-3细胞Ca2+]i浓度。结果:①10-5mol/L浓度的BBS可促进PC-3细胞CK表达及伪足形成;②BBS可提高PC-3细胞Ca2+]i浓度,并具有浓度依赖性。结论:实验证明一定浓度的BBS可明显提高PC-3细胞Ca2+]i浓度及CK表达,进而影响PC-3细胞骨架形态。本研究为探索BBS应用于肿瘤研究以及BBS作用后细胞内信息传递途径提供了基础。

Bombesin Increases Cytokeratin Expression and Free Calcium Concentration in PC-3 Cell Line

Objective: To observe the effect of bombesin on cytoskeleton form and intracellular free calcium(Ca~2+]i) concentration in PC-3 prostate cancer cell line. Methods: Immunofluorescent histochemistry (IH) combined with laser scanning confocal microscopy(LSCM) was used to examine the expression of cytokeratin(CK) in PC-3 cells treated with definite concentrations of BBS and observe its effect on cytoskeleton form. Fluo-3/AM fluorescence technique and LSCM were adopted to measure the Ca~2+]i concentration after different concentrations (10~-9, 10~-7 and 10~-5mol/ L) of BBS were added in PC-3 cells. Results: BBS(10~-5mol/L) stimulated the expression of CK in PC-3 cells and the formation of lamellipodium, and increased the Ca~2+]i concentration, with concentration dependence. Conclusion: Definite concentrations of BBS could obviously enhance the Ca~2+]i concentration, CK expression and cytoskeleton morphology of PC-3 cells. The results provide a basis for further studies on the role of BBS in tumour researches as well as in intracellular signal transmission.