| 离子交换色谱法测定枳实中辛弗林含量 |
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| 引用本文： | 陈燕军,吴有根.离子交换色谱法测定枳实中辛弗林含量[J].亚太传统医药,2013,9(6):46-47. |
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| 作者姓名： | 陈燕军 吴有根 |
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| 作者单位： | 1. 江西师范大学,江西南昌,330027
2. 南昌职业学院,江西南昌,330004 |
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| 摘 要： | 目的：建立枳实中辛弗林的含量测定方法。方法：采用离子交换色谱法，色谱柱Waters SCX柱（4．6mm×250mm，5μm）；流动相：乙腈-60mmol／L磷酸二氢钾（10：90）；流速：1．0mL／min；柱温：25℃，检测波长：275nm。结果：辛弗林进样量在0．1908-1．5264μg范围内与色谱峰面积线性关系良好（r=0．9999），加样回收率为100．23%（RSD=1．93％，n=9）。该法具有较好的精密度、重复性和稳定性。结论：离子交换色谱法测定辛弗林含量，操作简便、准确，且重现性好。
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| 关 键 词： | 离子交换色谱法 枳实 辛弗林 |
Determination of Synephrine in Aurantii Fructus Immaturus by Ion Exchange chromatography |
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| Chen Yanjun
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Wu Yougen.Determination of Synephrine in Aurantii Fructus Immaturus by Ion Exchange chromatography[J].Asia-Pacific Traditional Medicine,2013,9(6):46-47. |
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| Authors: | Chen Yanjun Wu Yougen |
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| Institution: | 2 (1. Jiangxi Normal University,Nanchang 330027, China; 2. Nanchang University of Vocational Technologies,Nanchang 330004, China) |
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| Abstract: | Objective：To estabish a method for determination of synephrine in aurantii fructus immaturus by ion exchange chroma tography. Methods：Waters SCX（4.6mm×250mm,5μm） for column. Acetonitrile-60mmol/L KDP= 10 ： 90 for mobile phase, de tective wavelength： 275 nm , flow rate ： 1mL/min, temperature ： 25 ℃. Results： For sypephrine, the linear range was from 0.1908 - 1 5264μg（r=0. 999 9） , average recovery rate was 100.23 %, RSD= 1. 93 % （n= 9）, with precise accurate, recurrence and stability. Conclusion： Determining the quantity of sypephrine in aurantii fructus by ion exchange chromatography is simple, accurate and re producible |
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| Keywords: | Ion Exchange Chromatography Aurantii Fructus Synephrine |
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