乙型肝炎病毒感染者外周血T淋巴细胞亚群表达与其血清HBV-DNA水平的相关性分析

观察乙型肝炎病毒(HBV)感染者外周血T细胞亚群功能变化与其血清HBV-DNA水平,探讨病毒复制程度与细胞免疫功能的关系.应用流式细胞术直接荧光法检测142例HBV感染者和35名健康对照者外周血中T淋巴细胞亚群百分率,用荧光定量PCR法检测HBV感染者血清HBV-DNA.结果显示:与健康对照组比较,慢性乙型肝炎组CD4...

Relationship Between Serum HBV DNA Replication and T Lymphocyte Subsets of Peripheral Blood in Patients with Hepatitis B

To observe T lymphocyte subsets in peripheral blood in patients with hepatitis B and the replication of serum hepatitis B virus(HBV-DNA),to explore the relationship between the viral replication degree and cellular immune function.The relative percentage of T lymphocyte subsets in peripheral blood of 142 patients with hepatitis B and 35 healthy controls was analyzed by flow cytometry.The serum load of HBV-DNA was measured by fluorescence quantitative polymerase chain reaction(PCR).Compared with healthy controls,the percentage of CD4+ T cells in patients with chronic hepatitis B were significantly decreased and the percentage of CD8+ T cells were increased(P<0.01).Compared with healthy controls,the CD4+/CD8+ratio in patients with cirrhosis were decreased and the percentage of CD4+CD25+ regulatory T cells were increased(P<0.05).Chronic hepatitis B infection can lead to dysfunction of cellular immunity;HBV-DNA high replication status can further promote the cellular immune dysfunction.The dynamic detection of CD4+/CD8+ ratio can provide the basis for clinical immunotherapy.