| 白细胞介素-13对肾小球系膜细胞增殖及细胞周期的影响 |
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| 引用本文： | 王碧飞,陈孝文,江黎明,梁东,唐德燊. 白细胞介素-13对肾小球系膜细胞增殖及细胞周期的影响[J]. 中国中西医结合肾病杂志, 2003, 4(9): 503-505 |
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| 作者姓名： | 王碧飞 陈孝文 江黎明 梁东 唐德燊 |
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| 作者单位： | 广东医学院附属医院肾脏病研究所,湛江,524001 |
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| 基金项目： | 广东省自然科学基金资助项目 (No .0 0 15 3 4) |
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| 摘 要： | 目的：探讨白细胞介素—13(IL—13)对肾小球系膜细胞(MC)增殖及其细胞周期的影响。方法：用甲基偶氮唑法(MTT)测定MC增殖，流式细胞术分析细胞周期。结果：IL—13在1ng／ml、5ng／ml、10ng／ml、100ng／ml浓度范围呈剂量依赖性抑制5％FCS培养的和脂多糖(LPS)诱导的MC增殖，抑制率分别为19．8％和29．23％、24．35％和37．05％、30．03％和46．6％、46．93％和55．23％。IL—13作用MC48h，使MC较多滞留于G1期，而S期细胞减少。结论：IL—13通过参与细胞周期的调控而抑制MC增殖。
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| 关 键 词： | 白细胞介素-13 肾小球系膜 细胞增殖 细胞周期 肾小球疾病 |
| 修稿时间： | 2002-11-25 |
Effects of Interleukin-13 on Proliferation and Cell Cycle of Rat Mesangial Cells |
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| WANG Bifei,CHEN Xiaowen,JIANG Liming,et al Institute of Nephrology,Affiliated Hospital of Guangdong Medical College,Zhanjiang. Effects of Interleukin-13 on Proliferation and Cell Cycle of Rat Mesangial Cells[J]. Chinese Journal of Integrated Traditional and Western Nephrology, 2003, 4(9): 503-505 |
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| Authors: | WANG Bifei CHEN Xiaowen JIANG Liming et al Institute of Nephrology Affiliated Hospital of Guangdong Medical College Zhanjiang |
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| Abstract: | Objective:To study the effects of interleukin-13(IL-13)on proliferation and cell cycle of rat mesangial cells.Methods:Cell prolifration was measured by MTT method.Flow cytometry was used to analyse cell cycle distribution.Results:At the concentration of 1 ng/ml,5 ng/ml,10 ng/ml and 100 ng/ml,IL-13 inhibited the proliferation of 5% FCS-cultured and LPS-induced mesangial cells by 19.83% and 29.23%,24.35%and 37.05%,30.03%and 46.67%,46.93%and 55.53%,respectively.The percentage of mesangial cells in G 1 phase markedly increased,while the percentage of mesangial cells in S phase markedly decreased after 48 h incubation with IL-13.Conclusion:IL-13 inhibit proliferation of mesangial cells.The inhibitory mechanism is at least partially through regulating cell cycle. |
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| Keywords: | Interleukins Kidney glomerulus Cell cycle Proliferation |
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