光动力联合塞来昔布对子宫颈癌COX-2表达及影响的研究

目的研究光动力联合塞来昔布对子宫颈癌细胞中环氧合酶-2(cyclooxynase-2,COX-2)蛋白表达及细胞增殖和凋亡的影响。方法①培养子宫颈癌Hela细胞,应用四甲基偶氮唑蓝(MTT)比色法检测选择性COX-2抑制剂塞来昔布(celecoxib)对子宫颈癌Hela细胞增殖抑制作用。②将Hela细胞分为4组:空白对照组(Hela,H组)、塞来昔布组(celecoxib,C组)、光动力组(photodynamics therapy,PDT,P组)、塞来昔布+光动力组(celecoxib+photodynamics therapy,P+C组)、分别应用蛋白印迹法(Westernblot)和免疫组化法检测COX-2蛋白表达情况。结果 MTT比色法检测显示,celecoxib作用Hela细胞24 h后对宫颈癌细胞有相对的抑制作用。Western blot和免疫组化法检测结果均显示:celecoxib联合PDT作用Hela细胞24 h后COX-2蛋白的相对表达水平明显降低,与其它3组相比,差异有统计学意义(P<0.05)。结论选择性COX-2抑制剂celecoxib能抑制人子宫颈癌细胞的增殖,且呈浓度依赖性,随着浓度的增大作用逐渐增强。PDT联合选择性COX-2抑制剂celecobix能有效抑制靶基因COX-2的表达,进而可抑制子宫颈癌细胞增殖并诱导细胞调亡增加,为子宫颈癌的基因研究及治疗提供新思路。

Study on the effects and expression of cyclooxynase-2 of photodynamics therapy combined with celecoxib of cervical carcinoma

Objective To study the effects and expression of cyclooxynase-2 of photodynamics therapy combined with celecoxib on the proliferation and apoptosis in cervical carcinoma cells.Methods ① Hela cells were cultured,the proliferation and suppressive effects of using celecoxib in cervical cancer cell were detected by MTT.② Hela cells were divided into control(H),celecoxib(C),PDT(P) and PDT+celecoxib(P+C) group.The cyclooxygenase-2 protein expression was detected by Western blot and immunohistochemistry technique.Results For 24 hours,cervical carcinoma cells was suppressed relatively by MTT.Photodynamics therapy combined with celecoxib for 24h,the protein expression of cyclooxygenase-2 was suppressed after,more compared with other three groups,the difference was significantly(P<0.05).Conclusion Celecoxib could suppress proliferation of cervical carcinoma cells,and the stronger density,the obvious restrain.Photodynamics therapy combined with celecoxib can effectively inhibit the expression of target gene cyclooxygenase-2 and might be potentially useful in gene therapy of cyclooxygenase-2 related cervical carcinoma.Cyclooxygenase-2 gene can suppress the proliferation and promote the apoptosis in Hela cells.