成人急性髓细胞白血病异常免疫表型特点及临床意义

目的探讨急性髓细胞白血病(acute myelocytic leukemia,AML)患者的异常免疫表型特点及临床意义。方法采用多色流式细胞术对初诊的38例AML患者进行白血病免疫表型检测。结果 AML患者髓系特异性抗原表达阳性率高低依次为:CD33、CD13、cMPO、CD117、CD11b;部分患者异常表达淋巴系抗原,表达率高低依次为CD19、cCD79a、CD7等。CD19+、CD7+患者完全缓解(complete remission,CR)率低于CD19-、CD7-患者,差异有统计学意义(P<0.05);部分患者表达非特异性早期抗原CD34、HLA-DR、CD38,CD34+患者CR率与CD34-患者CR率相比差异无统计学意义(P>0.05),CD56+患者CR率与CD56-患者CR率相比差异无统计学意义(P>0.05)。结论 CD33、CD13、cMPO、CD117为髓系常见且敏感的抗原。伴有CD19、CD7表达的患者,化疗后完全缓解率低于阴性者。

Characteristics of abnormal immunophenotype and clinical significance in acute myelocytic leukemia

Objective To investigate the characteristics of abnormal immunophenotype and clinical significance in acute myelocytic leukemia(AML).Methods Immunophenotyping of 38 adults with AML was performed by multi-color flow cytometry analysis.Results Myeloid-associated specific antigen of AML patients from high to low were: CD33,CD13,cMPO,CD117,CD11b.Cross-lineage antigen expression from high to low were: CD19,cCD79a,CD7 and so on.CD19+,CD7+ patients had lower complete remission(CR) rate than CD19-,CD7-patients(P<0.05).A proportion of patients had early nonspecific antigen expression.They were CD34,HLA-DR,and CD38.The CR rates were not statistically significant(P>0.05) between patients with CD34+,CD56+ and those with CD34-,CD56-.Conclusion CD33,CD13,CD117,and cMPO are common and sensitive myeloid associated antigens.The patients with CD19+ or CD7+ have lower CR rate than negative patients.