| HPLC法测定双氯芬酸钠水凝胶中双氯芬酸的含量 |
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| 引用本文： | 高晓波,张洪. HPLC法测定双氯芬酸钠水凝胶中双氯芬酸的含量[J]. 郧阳医学院学报, 2011, 30(1): 75-77 |
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| 作者姓名： | 高晓波 张洪 |
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| 作者单位： | 高晓波,GAO Xiao-bo(武汉大学人民医院药学部,湖北,武汉,430060;华中科技大学同济医学院附属荆州医院药学部,湖北,荆州,434020);张洪,ZHANG Hong(武汉大学人民医院药学部,湖北,武汉,430060) |
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| 摘 要： | 目的:建立HPLC法,测定双氯芬酸钠水凝胶中双氯芬酸的含量,用于医院内制剂的质量标准研究。方法:采用高效液相色谱法,以Kromasil ODS(250 mm×4.6 mm,5μm)为分析色谱柱;流动相:甲醇-pH 2.5磷酸盐缓冲液(70∶30);流速:1 mL.min-1;检测波长:254 nm;柱温为室温。结果:双氯芬酸在0.05～0.80 mg.mL-1浓度范围内具有良好的线性关系。结论:该方法可准确、快速、简便地测定双氯芬酸钠水凝胶中双氯芬酸的含量。
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| 关 键 词： | 高效液相色谱法 双氯芬酸钠 水凝胶 含量测定 |
Determination of Diclofenac in Diclofenacsodium hydrogel by HPLC |
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| GAO Xiao-bo,ZHANG Hong. Determination of Diclofenac in Diclofenacsodium hydrogel by HPLC[J]. Journal of Yunyang Medical College, 2011, 30(1): 75-77 |
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| Authors: | GAO Xiao-bo ZHANG Hong |
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| Abstract: | Objective:To establish the HPLC method for the determination of diclofenac in diclofenacsodium hydrogel for further specification studies of the hospital preparations.Methods The determination was performed with Kromasil ODS(250 mm×4.6 mm,5 μm) column with the flow rate of 1.0 mL·min-1.The mobile phase is methanol-pH 2.5 phosphate buffer(70:30).The detective wavelength is 254 nm.And the column temperature is room temperature.Results Diclofenac appears linear in the concentration range of 0.06 mg·mL-1 to 0.14 mg·mL-1 with high specificity.Conclusion This method could exactly,quickly and conveniently determine the diclofenac in diclofenacsodium hydrogel. |
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| Keywords: | HPLC Diclofenacsodium Hydrogel Content determination |
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