Improved B Cell Typing for HLA—DR Using Nylon Wool Column Enriched B Lymphocyte Preparations |
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| Authors: | R. Lowry J. Goguen C. B. Carpenter T. B. Strom M. R. Garovoy |
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| Affiliation: | Tissue Typing Laboratory, and the Howard Hughes Medical Institute Laboratory, Renal Division, Department of Medicine, Peter Bent Brigham Hospital, Boston, Mass., U. S. A. |
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| Abstract: | ![]()
A rapid, simple procedure for preparing B lymphocytes using nylon wool has been devised. When mixtures of T lymphocytes, B lymphocytes, and monocytes are applied to nylon wool columns, B lymphocytes can then be removed, virtually free of monocytes. Such preparations (Ad) are 85 +/- 6% SIg+ and have only 3 +/- 1% monocyte contamination. The yield from peripheral blood averages 5% of all mononuclear cells. In contrast, the E rosette depletion method (E-) yields cells which are only 64 +/- 5% SIg+ and have 25 +/- 7% monocyte contamination. The superiority of the nylon method for HLA-DR typing was demonstrated in a comparative study of Ad and E- with eight normal individuals. Cytotoxic scores were higher and a large number of reactions, representing DRw groups and additional cross-reactions, was detected. |
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