选择性COX2抑制剂对胃癌细胞增殖的影响

背景与目的：越来越多证据表明非甾体类抗炎药（NSAIDs)可以减少消化道肿瘤的危险,其化学预防作用在于抑制了环氧全酶－2（COX－2）,但选择性COX－2抑制剂对胃癌细胞生长的影响及机制目前3仍不十分清楚。本研究目的是观察选择性COX－2抑制剂尼美舒利对SGC7901胃腺癌细胞PGE2释放及增殖和凋亡的影响。方法：采用MTT比色法和放射免疫分析法观察尼美舒利对SGC7901细胞增殖及前列腺素E（PGE2）释放的影响,采用透射电镜及流式细胞仪观察尼美舒利对SGC7901细胞诱导凋亡的作用及细胞周期的影响。结果：尼美舒利呈时间,剂量依赖性方式抑制SGC7901细胞增殖,减少PGE2释放;改变细胞周期的分布,增加G0／G1期细胞比例,呈剂量依赖性非线型方式诱导其凋亡。结论：尼美舒利可能通过减少PGE2释放,影响细胞周期分布和诱导细胞凋亡,从而抑制SGC7901胃腺癌细胞的增殖,选择性抑制COX－2发挥其抑制人胃癌细胞恶性增殖和诱导凋亡的作用。

Influence of selective cyclooxygenase-2 inhibitor on proliferation of human gastric cancer cells

Background &Objective:Accumulating evidence indicates that nonsteroidal antiinflammatory drugs (NSAIDs) may reduce the risk of digestive system tumors, their chemopreventive effects appear to be due to inhibition of cyclooxygenase 2 (COX 2). At present, influence of selective COX 2 inhibitor on proliferation of human gastric cancer cells and mechanism were not very clear. The current study was designed to evaluate the potential role of Nimesulide, a selective COX 2 inhibitor, on the PGE2 level, proliferation and apoptosis of gastric adenocarcinoma cell line SGC7901. Methods: MTT assay and radioimmunoassay were used to determine the influence of Nimesulide on the proliferation of SGC7901 cells and PGE2 release in the culture supernatant; Transmission electron microscopy and flow cytometry were used to observe the induction of Nimesulide on the apoptosis of SGC7901 cells and influence on the distribution of cell cycle. Results: Nimesulide inhibited the cells proliferation in a time and dose dependent fashion and reduced the release of PGE2, induced apoptosis of the cells in a dose dependent and non linear manner and increased the proportion of cells in the G0/G1 phase. Conclusion:Nimesulide may inhibit the proliferation of gastric adenocarcinoma cells SGC7901 through decreasing PGE2 release, and affecting the distribution of cell cycle and inducing apoptosis. Selective COX 2 inhibitor may be a new way of the chemoprevention and chemotherapy for gastric carcinoma.