硫代反义Bcl-xL寡脱氧核苷酸对BcaCD885颊癌细胞凋亡及热敏感性的影响

目的　探讨硫代反义Bcl-xL寡脱氧核苷酸(AS-sODN)对BcaCD885颊癌细胞凋亡及热敏感性的影响。方法　以脂质体Lipofectin为载体,转染AS-sODN于BcaCD885细胞内,倒置显微镜观察细胞形态学改变,TUNEL原位末端标记检测细胞凋亡,流式细胞技术(FCM)测定细胞凋亡率及Bcl-xL蛋白表达水平;转染AS-sODN于BcaCD885 细胞内,再以43℃,40 min加热细胞,FCM检测细胞凋亡率。结果　AS-sODN转染后,BcaCD885颊癌细胞出现皱缩、呈浮起状,TUNEL原位末端标记阳性,Bcl-xL蛋白表达明显下降(P<0·05),热诱导细胞凋亡率明显提高(P< 0·05)。结论　硫代反义Bcl-xL寡脱氧核苷酸能诱导BcaCD885颊癌细胞凋亡并能提高其热敏感性。

Effect of Phosphorothioate Antisense Bcl-xL Oligodeoxynucleotides on Apoptosis and Thermosensitivity of BcaCD885 Cells

OBJECTIVE: To study the effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells. METHODS: After phosphorothioate antisense Bcl-xL oligodeoxynucleotides were transfected into BcaCD885 cells. The characteristics of apoptotic cells were evaluated by morphological observation and TUNEL staining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermia with 43 degrees C 40 min was also examined through flow cytometry. RESULTS: The BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apoptotic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P < 0.05). The apoptotic rate of these cells induced by hyperthermia was increased significantly compared with the controlled group (P < 0.05). CONCLUSION: Phosphorothioate antisense Bcl-xL oligodeoxynucleotides can induce apoptosis and improve thermosensitivity of BcaCD885 cells.