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Reactivity of Haemophilus influenzae type b anti-pili antibodies
Authors:J R Gilsdorf  L J Forney  J J LiPuma
Affiliation:Department of Pediatrics and Communicable Diseases, University of Michigan Medical Center, Ann Arbor.
Abstract:
The reactivity of anti-pilus antibodies to native and denatured Haemophilus influenzae b (Hib) pili was studied using rabbit serum prepared against piliated H. influenzae b strain M43 (p+) and adsorbed with its non-piliated variant, strain M42 (p-). The specificity of the adsorbed serum for Hib pili was documented by immunogold electron microscopy and by immunoprecipitation, which revealed the 24 kDa pilin band from strain M43 (p+) that was not seen on strain M42 (p-). In immunodot assays, the anti-pilus antibodies reacted with the native pili present on the outer membrane of strain M43 (p+), but on Western blot assay using denatured outer membranes, the anti-pilus antibodies did not react with the 24 kDa pilin subunit. These data demonstrate that the anti-pilus antibodies in the adsorbed serum recognize conformational epitopes that depend on the tertiary or quaternary structure of Hib pili.
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