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| RNA Interference-Mediated Gene Silencing of Vascular Endothelial Growth Factor | |
| 引用本文： | Tiejun Li Jianning Song Kai Kang Zanlan Hu Tongchuan He Bingqiang Zhang Caiquan Zhang. RNA Interference-Mediated Gene Silencing of Vascular Endothelial Growth Factor[J]. 中国肿瘤临床(英文版), 2006, 3(6): 408-412. DOI: 10.1007/s11805-006-0129-6 |
| 作者姓名： | Tiejun Li Jianning Song Kai Kang Zanlan Hu Tongchuan He Bingqiang Zhang Caiquan Zhang |
| 作者单位： | Tiejun Li1，2 Jianning Song2 Kai Kang1 Zanlan Hu2 Tongchuan He3 Bingqiang Zhang1 Caiquan Zhang1 1 The First Affiliated Hospital，Chongqing University of Medical Sciences，Chongqing，400016，China. 2 The Ninth Hospital of Chongqing，Chongqing，400700，China. 3 Molecular Oncology Laboratory，The University of Chicago Medical Center，Chicago IL 60637，USA. |
| 摘 要： | A ngiogenesis is a well-known process that is essential for tumor growth beyond 2 mm.[1] Although numerous growth factors are involved, vascular endothelial growth factor (VEGF), in particular VEGF-A, has been shown to play an important role in tumor angi… |
| 关 键 词： | 脉管 RNA 肿瘤 细胞 血管 |
| 收稿时间： | 2006-11-27 |
| 修稿时间： | 2006-12-08 |
RNA interference-mediated gene silencing of vascular endothelial growth factor |
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| Tiejun Li,Jianning Song,Kai Kang,Zanlan Hu,Tongchuan He,Bingqiang Zhang,Caiquan Zhang. RNA interference-mediated gene silencing of vascular endothelial growth factor[J]. Chinese Journal of Clinical Oncology, 2006, 3(6): 408-412. DOI: 10.1007/s11805-006-0129-6 | |
| Authors: | Tiejun Li Jianning Song Kai Kang Zanlan Hu Tongchuan He Bingqiang Zhang Caiquan Zhang |
| Affiliation: | 1. The First Affiliated Hospital, Chongqing University of Medical Sciences,Chongqing, 400016, China;The Ninth Hospital of Chongqing,Chongqing, 400700, China 2. The Ninth Hospital of Chongqing,Chongqing, 400700, China 3. The First Affiliated Hospital, Chongqing University of Medical Sciences,Chongqing, 400016, China 4. Molecular Oncology Laboratory, The University of Chicago Medical Center,Chicago IL 60637, USA. |
| Abstract: | OBJECTIVE To inhibit the expression of the vascular endothelial growth factor (VEGF) by RNA interference, and to observe the effect in different cells line. METHODS Using the services of E-RNAi, we designed and constructed two kinds of shRNAs expression vectors which were aimed at the VEGF gene. These vectors were then transfected into HEK293, colon cancer HT29, Hela and HepG2 cells by LipofectamineTM 2000. The level of VEGF mRNA was determined by RT-PCR and Northern blotting and the VEGF expression was examined by immunofluoresence staining. RESULTS The two kinds of VEGF specific shRNAs expression vectors were found to efficiently inhibit the expression of VEGF in HEK293 and HT29 cells by RT-PCR analysis, with inhibition rates of 72% and 42%, respectively; but the inhibition rates were reduced to 28% in Hela cells and 13% in HepG2 cells. Northern blotting showed that the inhibition rates of VEGF mRNA expression were 88% and 89% in HEK293 and HT29 cells, respectively. The inhibition rate of VEGF protein expression in HT29 cells was 73% based on immunofluoresence staining. CONCLUSION The expression of VEGF was inhibited by RNA interference, but differed with various cells lines, showing that RNA interference was cell-line dependent. |
| Keywords: | RNA interference VEGF tumor cells Northern blotting. |
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