乙型肝炎病毒DNA与血清免疫标志物的关系分析

目的 探讨乙型肝炎病毒DNA(HBV DNA)与血清免疫标志物的关系.方法 荧光定量聚合酶链反应法 (FQ-PCR) 检测血清样本中HBV DNA含量;酶联免疫吸附法(ELISA)检测HBV血清标志物.结果 A组(HBsAg和HBeAg阳性)、B组(大三阳)、C组(HBsAg 、HBcAb阳性)和D组(小三阳)的HBV DNA阳性率分别为96.7%、87.6%、52.2%和38.2%,各组之间均有显著性差异(P＜0.05); A组中阳性样本的HBV DNA量显著高于其它组别(P＜0.05); 315份HBV DNA阳性标本全部携带有HBsAg;HBsAg( )HBeAg( )和HBsAg( )HBeAg(-)中HBV DNA阳性率分别为89.1%和44.0%,有显著性差异(P＜0.05).结论 HBsAg是HBV感染灵敏的血清标志物;FQ-PCR定量检测HBV DNA作为血清学方法的补充对乙肝的临床诊断具有重要意义.

Relationship between HBV DNA and serum markers

Objective To explore the relationship between serum HBV markers and HBV DNA in serum. Methods HBV DNA in the serum was determined by FQ-PCR and serum HBV markers tested by ELISA method.Results The level of HBV DNA in group A (HBsAg and HBeAg positive) was significantly higher than in groups B and C (P<0.05); All samples with HBV DNA( ) were also positive for HBsAg. Respectively, 89.1% and 44.0% in the group of HBsAg( )HBeAg( ) and the group of HBsAg( )HBeAg(-) were HBV DNA( ). There was significant difference between the two groups.Conclusion HBsAg is the sensitive serum marker for HBV infection;FQ-PCR has the important clinical value as a supplement of serum detected methods for diagnosis of hepatitis B.