外源p53基因和TNFa基因转导对膀胱癌细胞在裸鼠体内的生长抑制

通过逆转录病毒载体将野生型ｐ５３基因、ＴＮＦａ基因单独或与ＩＬ－６基因连接（ＴＮＦＩＬ－６）分别导入膀胱癌细胞株ＥＪ和ＢＩＵ－８７．裸鼠致瘤试验和体外生长实验显示：ｐ５３基因转染组细胞接种裸鼠后９周无肿瘤生长，体外生长速率明显降低，Ｎｏｒｔｈｅｒｎ杂交显示Ｈ－ｒａｓ基因表达明显低于非转染组细胞，ＴＮＰａ基因和ＴＮ刊ＩＬ－６基因转染组裸鼠瘤体积明显小于对照组，体外生长速率与对照组无显著差别，两种细胞因子对Ｈ－ｒａｓ基因表达无影响。

Suppression of Tumor Formation in Vivo in BALB/cNude Mice by Transfection of Genes Coding forWild-Type p53 and TNFa

etroviral vectors were used to introduce p53 gene. TNF-a gene orTNF-a/IL-6 genes into hnman bladder cancer cells ( E-J. BIU-87 ) . Success-ful gene integration and expression were determined by Northern hybridization.Different levels of TNFa activity of the cultnre supernatant of the cellstransduced with TNFa gene were detected by L929 bioassay.The growth rateof the tumor cells was not significantly suppressed by TNFa gene transfer,but tumor formation in BALB/c nude mice was obviously delayed, in whichthe effects revealed no difference between TNFa producers and TNFa/IL-6producers. No tumors was found in BALB/c nude mtce 9 weeks afterp53 gene-transduced cell inoculations. Expression of H-ras mRNA of the tu-mor cells transduced with p53 gene was significantly lower than the controls.