磷酯酶C对家兔血小板聚集和超微结构的影响

目的　应用电镜研究磷酯酶C(phospholipaseC ,PLC)对家兔血小板聚集和超微结构的影响 ,以探讨用药后PLC抗血小板聚集作用的机制。方法　家兔颈动脉插管取血 ,制备PRP ,将其分为 4组 :①空白对照组 ;②生理盐水组 ;③ 0 5UPLC组 ;④ 2 5UPLC组。 2～ 4组分别用ADP诱导聚集 ,测出其聚集抑制率 ,各组分别制成超薄切片样品进行电镜观察、摄片。结果　 0 5、2 5UPLC明显抑制血小板聚集反应 ,聚集抑制率分别为 86 0 3 %± 12 6%和 82 47%±5 49% ;0 5UPLC抑制血小板形成伪足 ,α颗粒和致密颗粒较多 ,OCS管腔较小 ,其超微结构较生理盐水组变化小 ;2 5UPLC处理血小板的形态结构和超微结构与空白对照组无差异 ,血小板呈圆形或椭圆形 ,边缘光滑 ,无伪足样突起 ,α颗粒、致密颗粒、OCS等正常分布于胞质中。结论　PLC明显抑制ADP诱导的血小板聚集反应及其超微结构的改变 ,这可能是PLC抗血小板聚集作用的重要原因之一

The effects of PLC on the ultrastructure of platelets

AIM To investigate the effects of PLC on ultrastructure of platelets. METHODS The effects of PLC on ADP induced platelet aggre-gation were detected by tubidmetry; the ultrastru-cture changes of platelet were analyzed by electron microscope. RESULTS The rate of PLC inhibited significantly platelet aggregation by ADP induced is 86 03%±12 06% and 82 47%±5 49%. Pseudopodia can inhibit by 0 5 U PLC, at this group increase in the number of the granules associated with enhanced intensities of their electron densities and smaller dilated canalicular system. compared with normal saline group. In the 2 5 U PLC group the platelet morphology and ultrastructure approach blank group. The platelet is very smooth. PLC can inhibit producing pseudopodia-like process and the releasing of granules. Dilated canalicular system is similar to blank group, But it was different to normal group. CONCLUSION It's showed that PLC can significantly inhibit platelet aggregation and ultrastructural changes.