CD226在系统性红斑狼疮患者T淋巴细胞亚群上的表达

目的：研究CD226在SLE患者外周血T淋巴细胞亚群上的表达,以阐明CD226抗原在SLE患者体内T细胞活化中作用以及与SLE发病的关系。方法：31例SLE患者和30例健康志愿者外周血单个核细胞,在体外培养72h后,三色荧光标记的单克隆抗体染色,利用流式细胞仪测定T细胞亚群细胞表面CD226抗原的表达。结果;SLE患者组的CD3^ 、CD4^ 、CD8^ T淋巴细胞上CD226^ 表达均高于正常对照组（P＜0．01）;活动期SLE组、静止期SLE组的CD3^ 、CD4^ 、CD8^ T细胞上CD226^ 表达均显著高于对照组（P＜0．01）,而活动期与静止期患者之间T细胞亚群上CD226^ 表达则无显著性差异（P＞0．05）。SLE患者CD3^ 、CD4^ 、CD8^ T细胞CD226^ 抗原表达水平与SLEDAI之间无明显相关性（P＞0．05）。结论：SLE患者体内存在T细胞亚群异常活化;活动期、静止期SLET淋巴细胞CD226^＋表达均增高,CD226^ 可能参与了SLE的免疫发病。

Expression of CD226 on T lymphocyte subsets from patients with systemic lupus erythematosus

Objective Expression of the CD226 in T cell subsets from patients with systemic lupus erythematosus(SLE) was measured. To investigate whether function of CD226 antigen in T cell subsets activation process from patients with SLE is correlated with the pathogenesis of SLE. Methods Peripheral blood mononuclear cells from 31 SLE and 30 healthy volunteers were quantified by three colours immuno fluorescent staining method and using flow cytometric analysis. Results Percentage of CD226 positive from SLE on CD3 +,CD4 + and CD8 + cells increased, as compared with that found in the control group( P <0.05). The expression of CD226 on CD3 +,CD4 + and CD8 + T cells from active and inactive SLE were all significantly higher than that found in the control group( P <0.05). However, there was no difference between that from active SLE and that from the inactive SLE ( P >0.05).Conclusion There are some abnormal activated T cell subsets in SLE. The expression of CD226 + on CD3 +,CD4 + and CD8 + T cells with SLE increased. So CD226 may play an important role in the mechanisms of immune activation and immunity mechanism in SLE.