VITEK-2 Compact细菌自动化鉴定与药敏仪器检测碳青霉烯类耐药肠杆菌科细菌的性能评价

目的 评价VITEK-2 Compact细菌自动化鉴定与药敏仪器对碳青霉烯类耐药肠杆菌科细菌(CRE)的鉴定能力. 方法 同时采用PCR法和VITEK 2 Compact系统检测本实验室保存的93株CRE.以PCR结果为标准,评价VITEK-2 Compact仪器对CRE菌株的鉴定能力. 结果 VITEK-2 Compact细菌自动化仪器检测CRE的灵敏度为76.4％、特异性为34.2％、阳性预测值为62.7％、阴性预测值为50.0％. 结论 VITEK-2Compact细菌自动化仪器检测CRE的灵敏度有待提高,需结合其他方法给予补充,以提高该仪器的鉴定率与准确率.

Performance Evaluation of VITEK-2 Compact Automated Systems Detecting Carbapenemase-Resistant Enterobacteriaceae

ObjectiveTo evaluate the performance of VITEK-2 Compact Automated Systems for the detection of carbapenemase-resistant Enterobacteriaceae(CRE).Methods93 CRE stored in our laboratory were used in this study.The bacterial identification and antibiotic susceptibility tests were performed by VITEK-2 Compact automated system.Some common carbapenem-resistant genes were amplified by PCR, and the positive PCR products were sequenced to determine genotypes.With the PCR results as the standard, we evaluated the performance of VITEK-2 Compact Automated Systems for the detection of carbapenemase-resistant Enterobacteriaceae.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of VITEK-2 Compact to detection CRE were 76.4%, 34.2%, 62.7%, and 50.0%, respectively.ConclusionThe sensitivity of the VITEK-2 Compact Automated Systems for detection of CRE needs to be improved.In order to enhance the identification rate and accuracy of the instrument, the laboratory should complement other methods.