神经活性物质对小鼠学习记忆能力及耐力的影响

目的：观察神经活性物质（胚脑组织提取物，NAF）对小鼠学习、记忆能力提高的作用，以及对小鼠耐力的影响。方法：采用随机分组法，将51只小鼠按体重等分，分为5个实验组：生理盐水组（A）9只、脑活素组（B）11只和3个不同浓度（C：0．1mg,10只、D：0．2mg，10只、E：0．4mg ,11只）的NAF治疗组.用自制的复杂迷宫检测小鼠的迷宫觅 食时间及行走的错误次数，测定小鼠负重5％体重游泳至淹死的时间。结果：迷宫觅食时间A组治疗前后无明显差异（P＞0．01），B、C、D、E组治疗后缩短或明显缩短（P＜0．05－0．001）；治疗前后小鼠行走的错误次数A组和B组无显著性差异（P＞0．2－0．5），C、D、E组有非常显著性差异（P＜0．02－0．01）；小鼠负重5％体重游泳淹死时间5组间方差分析有显著性差异（P＜0．05）；进一步分析差异来自于C与E组（P＜0．05）和D与E组（P＜0．01）。结论：NAF可提高小鼠的学习、记忆能力，也可增强小鼠的耐力。腹腔注射≥20mg/kg 体重时，小 鼠的耐力反而下降。

Effect of Nerval Active Factors on learning,memory and stamina of mice

ObjectiveTo study the effect of extract of fetal brain (Nerval Active Factors, NAF) on learning, memory and stamina of mice.Methods51 mice were divided into five groups randomly: normal saline as Group A, Cerebrolysin as Group B, NAF as Group C: 0 1mg,Group D: 0 2mg,Group E: 0 4mg, i.p.The time looking for food and error frequency of walking road were tested by using complicated maze, and the drowning time was tested when the mice carried 5% body weight on its back.ResultsBefore and after treatment,the time looking for food in maze showed no significant change in Group A( P >0 1) and was significantly shorter in Group B, C, D and E( P <0 005, P <0 05, P <0 001, P <0 001).There was no significant difference on error frequency of walking road in Group A and B( P >0 2, P >0 5) before and after treatment, while it was significantly decreased in mice of Group C, D and E( P <0 02, P <0 02, P <0 01). There was significant difference on drowned time of mice carrying 5% body weight on back in five groups by analysis of variance (P<0 05) after treatment, but this difference derived from C vs E( P <0 05)and D vs E( P <0 01).Conclusions NAF can raise the activity of learning and memory of mice, as well as increase their stamina. On the other hand, the stamina of mice decreases when NAF is given more than 20 mg/kg body weight.