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| Effect of rhVEGF gene transfection on survival of grafts after autologous free granular fat transplantation in rats | |
| 引用本文： | 雷鸣 刘世清 彭昊 刘宇兰. Effect of rhVEGF gene transfection on survival of grafts after autologous free granular fat transplantation in rats[J]. 中华创伤杂志(英文版), 2008, 11(1): 49-53 |
| 作者姓名： | 雷鸣 刘世清 彭昊 刘宇兰 |
| 作者单位： | [1]Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan 430060, China [2]Department of Plastic ＆ Reconstructive Surgery, Third Hospital of Wuhan City, Wuhan 430060, China |
| 摘 要： | Objective： To investigate the effect of recombinant human vascular endothelial growth factor （rhVEGF） on autologous free granular fat grafts in rats. Methods： Forty-eight Sprague Dawley （ SD ） rats, weighing 190-280 g and regardless sex, were randomly divided into three groups, sixteen in each. After fat transplantation, the rats were treated with plasmid DNA encoding rhVEGF protein （the experimental group ）, plasmid DNA （ the negative group） and normal saline （ the blank control group ）, respectively. At 3, 7, 15 and 30 days after transplantation, the rats were killed and the grafts were weighed, respectively. Histopathological changes were evaluated. Microvessel density and the expression of VEGF were examined by immunohistochemical staining and Western blotting. Results： The weights of the negative and blank control groups were significantly reduced on the 7th, 15th and 30th days compared with those of the experimental group. The expression of VEGF and the microvessel density in the experimental group were significantly higher than the other two groups during the latter periods. Conclusion： The plasmid encoding VEGF can induce expression of VEGF and angiogenesis in fat grafts and reduce the absorption of free fat grafts. |
| 关 键 词： | 脂肪移植 脂肪移植物 血管内皮细胞 外科手术 |
| 收稿时间： | 2007-09-08 |
Effect of rhVEGF gene transfection on survival of grafts after autologous free granular fat transplantation in rats |
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| LEI Ming,LIU Shi-qing,PENG Hao,LIU Yu-lan. Effect of rhVEGF gene transfection on survival of grafts after autologous free granular fat transplantation in rats[J]. Chinese journal of traumatology, 2008, 11(1): 49-53 | |
| Authors: | LEI Ming LIU Shi-qing PENG Hao LIU Yu-lan |
| Affiliation: | 1. Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan 430060, China 2. Department of Plastic & Reconstructive Surgery, Third Hospital of Wuhan City, Wuhan 430060, China |
| Abstract: | OBJECTIVE: To investigate the effect of recombinant human vascular endothelial growth factor (rhVEGF) on autologous free granular fat grafts in rats. METHODS: Forty-eight Sprague Dawley (SD) rats, weighing 190-280 g and regardless sex, were randomly divided into three groups, sixteen in each. After fat transplantation, the rats were treated with plasmid DNA encoding rhVEGF protein (the experimental group), plasmid DNA (the negative group) and normal saline (the blank control group), respectively. At 3, 7, 15 and 30 days after transplantation, the rats were killed and the grafts were weighed, respectively. Histopathological changes were evaluated. Microvessel density and the expression of VEGF were examined by immunohistochemical staining and Western blotting. RESULTS: The weights of the negative and blank control groups were significantly reduced on the 7th, 15th and 30th days compared with those of the experimental group. The expression of VEGF and the microvessel density in the experimental group were significantly higher than the other two groups during the latter periods. CONCLUSION: The plasmid encoding VEGF can induce expression of VEGF and angiogenesis in fat grafts and reduce the absorption of free fat grafts. |
| Keywords: | Vascular endothelial growth factor Fat transplantation Fat graft absorption |
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