左旋肉碱对氧化应激损伤肾小管上皮细胞的保护作用

目的观察左旋肉碱对过氧化氢（H2O2）应激损伤人肾小管上皮细胞的保护作用，并探讨其可能机制。方法用H2O2作用于人肾小管上皮细胞系HK-2，建立肾小管上皮细胞氧化应激损伤模型；MTT法检测左旋肉碱预处理后HK-2细胞的活力；用酶化学法测定HK-2细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、过氧化氢酶（CAT）活性及总抗氧化能力（T—AOC）、丙二醛（MDA）；荧光显微镜观察及流式细胞仪测定细胞内活性氧（ROS）及HK-2细胞凋亡率。结果左旋肉碱预处理12h能抑制H2O2损伤所导致的HK-2细胞活力降低，增加细胞中SOD、GSH—Px和CAT含量，提高细胞T-AOC，降低细胞中MDA和ROS水平，抑制HK-2细胞凋亡。结论左旋肉碱对氧化应激所致的肾小管上皮细胞损伤具有保护作用，其机制可能与增强细胞抗氧化能力、减少自由基生成、抑制脂质过氧化反应及细胞凋亡有关。

Protective effect of L-carnitine on human kidney tubular epithelial cell line under oxidative stress

Objective To investigate the protective effect and mechanism of L-carnitine on human kidney tubular epithelial cell line damaged.Methods Human kidney tubular epithelial cell line(HK-2 cells) were pretreated with L-carnitine for 12 h and then injuryed by H2O2.The cell viality was evaluted by MTT assays.Enzyme activities including superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT),total antioxidative capacity(T-AOC) and malondialdehyde(MDA) were determined by biochemical methods.Intracellu...