高同型半胱氨酸血症兔动脉粥样硬化模型炎性介质的变化及意义

目的：探讨高同型半胱氨酸血症(HHcy)与动脉粥样硬化(AS)炎症反应的关系。方法：将20只雄性家兔随机分为对照组和实验组（每组10只），采用皮下注射DL-蛋氨酸制作HHcy模型，采用酶联免疫吸附法（ELISA）检测各组家兔血清同型半胱氨酸（Hcy）、IL-1β、IL-6和IL-8水平，并观察家兔主动脉病理学形态改变，免疫组化染色观察并计算核因子-κB(NF-κB)的阳性细胞数。结果：实验组血清Hcy、IL-1β、IL-6和IL-8水平明显高于对照组（P<0.05）；肉眼可见实验组家兔主动脉可见斑块形成，斑块部位的动脉壁增厚、变硬；镜下可见对照组主动脉血管内皮层内皮细胞及内弹力板连续规整，中膜平滑肌细胞排列整齐，实验组可见局部内皮细胞坏死脱落及内弹力板损伤断裂，中膜平滑肌细胞胞核固缩且排列紊乱；实验组NF-κB阳性细胞数明显高于对照组（P<0.05）。结论：炎性因子的过多分泌可能是HHcy促进AS的重要机制之一，而NF-κB在此过程中同样发挥了重要的介导作用。

Changes and significance of inflammation factors in model rabbit with artherosclerosis induced by hyperhomocysteic acid

Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1β,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-κB positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1β,IL-6 and IL-8 in experiment group were higher than those in control group(P<0.05).Artherosclerosis changes were found in the aorta in experiment group and the number of NF-κB positive cells in the aorta in experiment group were more than those in control group(all P<0.05).Conclusion The excessive secretion of inflammative factors is one of the mechanisms that HHcy causes artherosclerosis and NF-κB plays an importment mediation role.