二乙基对硝基苯磷酯酶2基因G/A148多态性与2型糖尿病及血脂的关系

目的：了解二乙基对硝基苯磷酯酶（PON）2基因G／A148多态性与中国北方地区人群2型糖尿病的相关性及其与血脂等的关系。方法：采用配偶对的病例－对照研究设计，用聚合酶链反应（PCR）－长度多态性的方法检测PON2基因G／A148多态性。结果：PON2基因G／A148多态性的基因型频率和等位基因频率在2型糖尿病组和对照组间差异无显著性意义。与非肥胖、无G等位基因相比，肥胖无G等位基因时，发生2型糖尿病的危害险度（OR）值为2．04；肥胖有G等位基因时，OR增至3．18。在对照人群中，含G等位基因组的总胆固醇（TC）、低密度脂蛋白（LDL）及载脂蛋白B（ApoB)水平均比AA基因型组高。G等位基因对高脂血症的OR为2．48，P＝0．5。结论：在中国北方地区人群中PON2基因G／A148多态性与肥胖有协同促进2型糖尿病发生的作用。在非糖尿病人群中，此多态性与总胆固醇等血脂水平显著相关。

An association study of paraoxonase gene G/A148 polymorphism with type 2 diabtets mellitus and blood lipids level in Chinese population

Objective To test the role of paraoxonase (PON) 2 gene in the pathogenesis of type 2 diabetes mellitus in Chinese population. Methods The PON2 gene G/A148 polymorphism was detected by polymerase chain reaction (PCR) and appropriate restriction enzyme (PCR RFLP) in 132 couples of type 2 diabetes spouse based controls. Results The genotype frequency and allele frequency were not different between the case and the control group. Compared with non obesity subjects without G allele, the obesity subjects without G allele and the obesity subjects with G allele had an OR for type 2 diabetes of 2.04, 3.18, respectively. In control group, the levels of total and low density lipoprotein cholesterol and apolipoprotein B (ApoB) were higher in the GA/GG genotype than that in the AA genotype and the OR of G allele for hyperlipidemia was 2.48, P =0.05. Conclusion There were synergistic effects between the PON2 gene G allele and obesity on type 2 diabetes mellitus. PON2 gene G/A148 polymorphism was significantly associated with total cholesterol level in non diabetic Chinese population.