雌、孕激素对去卵巢大鼠肝脏维生素D受体mRNA表达的影响

目的探讨雌、孕激素对去卵巢大鼠维生素D受体mRNA的影响。方法25只成年SD雌性大鼠,随机分为Sham组(假手术组)、OVX组(单纯去卵巢组)、OVX+E组(去卵巢+补充雌激素组)、OVX+P组(去卵巢+补充孕激素组)OVX+E+P组(去卵巢+补充雌、孕激素组),每组5只。喂养3个半月后处死,取肝脏检测1,25-二羟维生素D3受体mRNA。结果与去卵巢组比较:(1)雌激素对去卵巢大鼠肝脏1, 25-二羟维生素D3受体mRNA的表达有显著上调作用;(2)雌、孕激素联合用药组1, 25-二羟维生素D3受体mRNA的表达介于单用雌激素和孕激素之间;(3)孕激素对去卵巢大鼠肝脏1,25-二羟维生素D3受体mRNA的表达无显著上调。结论雌激素能促进去卵巢大鼠肝脏1, 25-二羟维生素D3受体的表达。

Effect of estrogen and progesteron on the expression of 1, 25-dihydroxyvitamin D receptors mRNA in the liver of ovariectomized rats

Objective To study the effect of estrogen and progesteron on the expression of dihydroxyvitamin D receptor (VDR) mRNA in the liver of ovariectomized rats. Methods Twenty-five adult female SD rats were randomly divided, with equal numbers, into sham-operated group (sham), ovariectomized group (OVX), ovariectomized group with estrogen treat- ment (OVX+E), ovariectomized group with progesteron treatment (OVX+P) and ovariectomized group with both estrogen and progesteron treatment (OVX+E+P). After 3 months and a half of feeding, all animals were killed to assess VDR mRNA by way of reverse transcriptase-PCR (RT-PCR). Results RT-PCR revealed marked increase in the band intensity corresponding to VDR mRNA product in Sham, OVX+E, and OVX+E+P groups. Conclusion Estrogen may increase the transcription level of VDR gene in the liver of ovariectomized rats.