| 高效液相色谱法测定左卡尼汀片的溶出含量 |
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| 引用本文： | 李春茹,马连晴,沈桂丹,周春梅. 高效液相色谱法测定左卡尼汀片的溶出含量[J]. 实用药物与临床, 2010, 13(6) |
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| 作者姓名： | 李春茹 马连晴 沈桂丹 周春梅 |
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| 作者单位： | 沈阳东宇药业有限公司,沈阳,110036 |
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| 摘 要： | 目的建立高效液相色谱法测定左卡尼汀片的溶出含量。方法采用氨基色谱柱(4.6mm×250mm,5μm);流动相:磷酸二氢钾溶液(6.81g/L,用氢氧化钠调pH值至4.7)∶乙腈(35∶65);流速1.0mL/min;柱温:30℃;检测波长:205nm;进样量:20μL。结果左卡尼汀在浓度0.189～0.756mg/mL的范围内,线性关系良好(r=0.9999),溶出度平均回收率为100.17%,RSD值为0.39%(n=9)。结论本方法简便易行,结果准确可靠,专属性强,适用于左卡尼汀片的溶出含量测定。
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| 关 键 词： | 左卡尼汀片 高效液相色谱法 溶出含量测定 |
Determination of levocarnitine tablets's dissolution by HPLC |
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| LI Chun-ru,MA Lian-qing,SHEN Gui-dan,ZHOU Chun-mei. Determination of levocarnitine tablets's dissolution by HPLC[J]. Practical Pharmacy and Clinical Remedies, 2010, 13(6) |
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| Authors: | LI Chun-ru MA Lian-qing SHEN Gui-dan ZHOU Chun-mei |
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| Abstract: | Objective To establish an HPLC method for the dissolution determination of the Levocarnitine tablets.Methods Samples were separated on NH2 column(4.6 mm×250 mm,5 μm)using the solution of monopotassium phosphate(6.81 g/L,with sodium hydroxide to adjust pH 4.7)-acetonitrile(35∶65)as the mobile phase at a flow rate of 1 mL/min.The column temperature was 30 ℃.The detective wavelength was 205 nm and the sample size was 20 μL.Results The levocarnitine had good liner relationship in 0.189~0.756 mg/mL(r=0.999 9),and the average recovery rate was 100.17%,RSD was 0.39%.Conclusion The method is simple,rapid,accurate and precise. |
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| Keywords: | Levocarnitine tablets HPLC Content determination of dissolution |
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