抗急性出血性结膜炎病毒受体单克隆抗体的制备与鉴定

用细胞保护试验、间接免疫荧光试验及流式细胞仪等方法，对采用细胞融合技术所得到的抗肠道病毒７０型（Ｅｖ７０）和柯萨奇病毒Ａ２４型变异株（ＣＡ２４ｖ）受体的单克隆抗体进行了研究，发现４株抗Ｈｅｌａ细胞膜受体的单克隆抗体能同时阻断Ｅｖ７０和ＣＡ２４ｖ某些毒株感染ＨｅＬａ细胞。这一结果提示ＥＶ７０和ＣＡ２４存在有相似的受体，对这两种不同的病毒能引起同一种临床疾病的发病机制的阐明有一定的指导意义。

Preparation and identification of monoclonal antibodies against cell receptors of acute hemorrhagic conjunctivitis viruses

To prepare monoclonal antibodies for blocking acute hemorrhagic conjunctivitis (AHC)viruses,BALB/C mice were immunized with Hela cells.The spleen of immunemouse was re-moved,and spleen cells were fused with SP2/0 cells.The antibodies against cell receptors wereselected by cell protection assay.Four cell strains secreted McAbs designated R7.R8.R16 andR24 were screened to be able to block cellular receptors and inhibit the infection of Ev70 as wellas CA24v on Hela cell.These results demonstrate that the receptors for Ev70 and CA24v are si-miliar.Indirect immunofluorescence test with the four McAbs showed definite fluorescence onliving Hela cells,which the intensity was concordance with the mean intensity resulted fromflowcytometer.Positive reaction on fixed Hela cells were only observed with R7 and R8.