前列腺素E1对糖尿病肾病模型大鼠肾上腺髓质素的影响

目的观察前列腺素E1对链腺佐茼素（STZ）诱导的糖尿病肾病（DN）模型大鼠肾上腺髓质素（ADM）的影响，初步探讨前列腺素E1治疗糖尿病肾病的作用机制。方法30只Wistar大鼠分为正常组、糖尿病肾病模型组、前列腺素E1组。前列腺素E1组予前列腺素E1200μg／（kg·d）进行干预。治疗4周、8周后检测24h尿蛋白定量、血清尿素氮、血清肌酐水平；用定量双抗夹心免疫吸附实验（ELISA）法检测ADM的表达。结果糖尿病肾病模型组24h尿蛋白排泄明显增加（P〈0．01），Scr水平上升（P〈0．05），ADM量增加；前列腺素E1干预与糖尿病肾病模型组比较，尿蛋白排泄明显减少，Scr水平降低，ADM表达减少。结论前列腺素E1对DN具有一定肾脏保护作用，其作用机制可能是通过降低ADM水平、减少尿蛋白而实现的。

Effect of PGE1 on expressions of adrenomedullin in rats with diabetic nephropathy

Objective To observe the effeets of PGEI on the expression of adrenomedullin in diabetic nephropathy（DN） rats,and to investigate the mechanism of PGEI for DN. Methods 30 Wistar rats were divided into normal eontrol group,DN group and treated group with PGE1 200μg/（ kg - d）. After 4 weeks and 8 weeks,serum ereatinine（Scr） and 24h urinary protein were both screened. The expression of ADM was examined by quantitative double-antibody sandwich enzyme-linked immunosorbent assay.（ELISA）. Results Compared with DN group ,the levels of Ser and 24h urinary protein exeretion,was reduced significantly decreased in PGE1 group（ P〈0. 05 or P〈0.01 ）. And the expression of ADM was significantly in PGE1 group（ P〈0. 05）. Conclusion PGE1 may be play a role in Reno proteetion in rats with DN, and it maybe eontribute to reduetion of urine protein by deereasing ADM.