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应用核酸分子杂交技术检测心肌炎组织中的肠道病毒RNA
引用本文:沈茜 汪美先. 应用核酸分子杂交技术检测心肌炎组织中的肠道病毒RNA[J]. 中华病理学杂志, 1994, 23(2): 79-81,T014
作者姓名:沈茜 汪美先
作者单位:上海第二军医大学附属长海医院临床实验诊断科
摘    要:
用生物素标记pCBIII/35和pCBIII/51肠道病毒属特异性探针,对45心肌炎和心肮病尸检或心肌活检心脏组织进行原位杂交,结果显示:12例(26.6%)存在肠道病毒RNA。柯萨基B3病毒型特异性探针pCBIII/29进一步杂交表明,12例经证实存在肠道病毒RNA的心肌组织中5例(41.7%)出现阳性杂交信号。阳性杂信号主要位于单个或多个心肌细胞的胞浆和心肌的间质组织,偶见竽心肌小血管内皮细胞

关 键 词:核酸杂交 心肌炎 肠道病毒 RNA

Nucleic acid hybridization technique detection of enterovirus RNA in myocarditis
Shen Qian, Wang Mei-xian,Zhang Tong-hua. Nucleic acid hybridization technique detection of enterovirus RNA in myocarditis[J]. Chinese Journal of Pathology, 1994, 23(2): 79-81,T014
Authors:Shen Qian   Wang Mei-xian  Zhang Tong-hua
Abstract:
orty five speciments from human myocarditic or myopathic tissues were examined for enteroviruses by in situ hybridization with 2 biotin lablled generic enterovirus probes pCBLII/35 and pCBIII/51.Pos-itive hvbridization signals were obtained in 12 samples(26.6%).Enterovirus RNA were obtained in 5 out of these 12 samples(41.7%) by the presence of positive hybridization signals with a coxsackie B3 virus typespecific probe pCBIII/29.The positive hybridization signals were primarily localizad within the cytoplasm of single or several myocytes and the myocardial interstitial tissues, and were also occasionally seen within the cytoplasm of endotheliocytes of blood vessels in the myocardium.
Keywords:Virus diseases Nucleic acid hybridization  
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