华蟾素对喉癌细胞凋亡的基础研究

目的 探讨中药华蟾素对体外培养人喉癌Ｈｅｐ－２细胞生长作用及癌基因变化。方法 应用光镜，电镜：ＭＴＴ检测技术；ＳＰ免疫组化技术及流式细胞分析仪，对药物作用后Ｈｅｐ－２细胞的数量，形态，抑癌基因Ｐ５３，癌基因ｃｍｙｃ，Ｂｃｌ－２蛋白的表达以及细胞周期ＤＮＡ含量的改变进行研究。结果 发现华蟾素对Ｈｅｐ－２细胞作用在低浓度短时间时促进细胞分裂、增殖和诱导细胞分化；高浓度长时间则抑制细胞生长，促进细胞凋亡

Basic Study of cell apoptosis for cinobufacini on human laryngeal carcinomacell

Objective To investigate the effects of cinobufacini on oncogenes in human la-ryngeal carcinoma cell line Hep- 2. Method In our experiment, the effect of cinobufacini on theproliferation of Hep-2 was observed by using the following methods: the morphologic changesof the cells were observed with optical and transmission electron microscopy, the cytotoxicity ofcinobufacini to these cell lines were measured by MTT assay, the expresser gene was determinedwith SP immunohistochem1stry, flow cytometry and image analysis. Result The results showedthat when Hep-2 cells were treated with cinobufacini for 24 hours, the cell proliferation was in-duced and cell became well -differentiated. When Hep -2 cells treated for 120 hours, the celldeath occurs by apoptosis. The expression of the oncogenes C-myc and Bcl-2 protein were re-markably decreased whereas that of the tumor suppresser gene P53 protein seems to increas.DNA content in the cycle progression of Hep - 2 cell was assessed by flow cytometry. lt wasfound that after Hep - 2 treated with cinobufacini 24 hours DNA synthesis was suppressed withsignificant GI block. Conclusion The antitumor effect and induce for the differentiation ofclnobufacini might be by Ineans of inhibiting the expression of oncogenes and improv1ng the ex-pression of carcinoma suppresser gene.[