p16基因上游序列与急性白血病细胞核基质蛋白结合的实验研究

目的探讨p16基因上游序列-869bp中是否含有核基质结合元件,并分析p16基因上游序列在急性白血病细胞组与对照组内与核基质蛋白结合的情况。方法DNA-蛋白质杂交(Southwestern blot)技术。结果p16基因上游序列与核基质蛋白的结合在急性白血病组与对照组间存在差异。结论p16基因外显子1α上游–869bp片段可以与核基质蛋白结合,表明该片段存在MAR序列,对p16基因表达调控具有重要意义,急性白血病细胞核基质蛋白成分的改变影响其与p16基因外显子1α上游–869bp片段MAR序列的结合,提示核基质蛋白成分的改变可能抑制p16基因的表达,促进急性白血病的发生。

The Study on the Binding of Upstream of p16 Gene and the Nuclear Matrix Protein in Acute Leukemia Cells

Objective To detect the change of nuclear matrix proteins in acute leukemic cells and the correlation between the upstream of p16 gene and nuclear matrix proteins in acute leukemia, and determine whether the 869bp fragment in the upstream of pl6gene include matrix attached region. Method DNA-protein blot（Southwestern blot）. Results The binding of upstream of p 16gene and NMPs between acute leukemia group and control group was significantly different. Conclusion The 869bp fragment in upstream ofexonl of p16gene can bind to NMPs. This indicate that the fragment include MAR sequence and can regulate p16gene expression. The changes of NMPs in acute leukemia can affect their binding with MAR sequence included in upstream of p16gene. This result may be inhibit p 16gene expression and accelerate acute leukemia development.