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| 大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究 | |
| 引用本文： | 赵宇,谢鹏,朱晓峰,蔡志友. 大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究[J]. 中国医科大学学报, 2010, 39(1): 14-17 |
| 作者姓名： | 赵宇 谢鹏 朱晓峰 蔡志友 |
| 作者单位： | 1. 重庆医科大学,附属第一医院神经内科,重庆,400016 2. 佳木斯大学,神经科学研究所,黑龙江,佳木斯,154007 |
| 基金项目： | 高等学校博士学科点专项科研基金资助项目(20050631012) |
| 摘 要： | 目的应用丝裂原激活的蛋白激酶激酶(MAPKK/MEK)特异性阻滞剂PD98059孵育大鼠神经干细胞(NSC),探讨MAPKK/MEK-MAPK/ERK信号通路在NSC中的作用。方法体外分离、培养大鼠NSC,应用不同浓度的PD98059孵育NSC后进行WTS-8、Nestin,BrdU、β-tubulin-Ⅲ、Western blot检测。结果PD98059对NSC的存活、增殖和分化的影响呈剂量依赖性,在PD98059较高浓度时NSC的存活率明显下降(P<0.05),BrdU和β-tubulin-Ⅲ的阳性细胞数明显少于正常对照组(P<0.05)。PD98059阻断ERK表达的作用呈剂量依赖性。结论MAPKK/MEK-MAPK/ERK信号通路对大鼠NSC的存活、增殖、分化起重要作用。 |
| 关 键 词： | 丝裂原激活的蛋白激酶激酶 细胞外信号调控激酶 神经干细胞 |
Effect of MAPKK/MEK-MAPK/ERK Signal Transduction Pathway in Rat Neural Stem Cells |
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| ZHAO Yu , XIE Peng , ZHU Xiao-fen , CAI Zhi-you. Effect of MAPKK/MEK-MAPK/ERK Signal Transduction Pathway in Rat Neural Stem Cells[J]. Journal of China Medical University, 2010, 39(1): 14-17 | |
| Authors: | ZHAO Yu XIE Peng ZHU Xiao-fen CAI Zhi-you |
| Affiliation: | ZHAO Yu1,XIE Peng1,ZHU Xiao-feng2,CAI Zhi-you1 (1. Department of Neurology,The First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China,2. The Neuroscience Research Institute of Jiamusi University,Jiamusi 154007,China) |
| Abstract: | Objective To incubate the rat neural stem cells with specific inhibitor(PD98059)of MAPKK/MEK to clarify the effect of MAPKK/MEK-MAPK/ERK signaling pathway in neural stem cells (NSC). Methods NSCs derived from E15-16 rats were isolated and cultured. After treated with different concentration of PD98059,they were subjected to the detection of cell proliferation,Nestin,BrdU and β- tubulin-Ⅲ by WTS-8 assay,immunofluorescent staining or Western Blot respectively. Results PD98059 had an effect on the survival,proliferation and differentiation of NSC at a concentration-dependent manner. The viability of the NSC was significantly decreased than the control(P < 0.05),whereas the numbers of BrdU-positive and β-tubulin-Ⅲ positive cells were notably fewer than the control(P < 0.05)after incubated with the higher concentration of PD98059. The ERK expression was blocked by PD98059 at a concentration-dependent manner. Conclusion The MAPKK/MEK-MAPK/ERK signaling pathway played a vital role in the survival,proliferation and differentiation of NSC. |
| Keywords: | mitogen activated protein kinases kinases extracellular signal regulated kinase neural stem cell |
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