慢性不可预见性温和应激后大鼠行为及微管相关蛋白tau磷酸化的改变

目的研究慢性不可预见性温和应激所致的动物行为学改变及微管相关蛋白tau磷酸化的改变。方法将大鼠随机分为慢性不可预见性温和应激抑郁（CUMS）组和对照组，对模型组大鼠进行连续21d的慢性不可预见性应激。进行行为学观察，使用western blotting检测总tau，tau磷酸化（Ser356，Thr231）水平的改变。结果CUMS组大鼠慢性应激后糖水偏好及自主活动显著减低，与对照组比较差异有统计学意义（P〈0．05）；CUMS组大鼠慢性应激后海马tau磷酸化表达升高，与对照组比较差异有统计学意义（P〈0．05）。结论慢性应激后微管相关蛋白tau磷酸化水平升高，神经可塑性受损，提示了新的抑郁症和阿尔茨海默病联系的生化机制。

Behavioral and tau protein alterations in rats following chronic unpredictable mild stress

Objective To investigate the behavior and hippocampal microtubule-associated proteins tau alterations. Methods Sixteen rats were divided into two groups, with 8 exposed to 21 conseeu tire days of chronic unpredicted mild stresses （CUMS） as depression models, and 8 as normal controls. Behavioral changes in these rats were analyzed in sucrose preference and open-field paradigm, and compared to a control group of normal rats. The expressions of hippocampat cytoskeletal microtubule-associated proteins p-tau were analyzed using Western Blot. Results （1） Compared with the normal rats, sucrose preference, total traveling distance, velocity and frequencies of rearing were reduced （P 〈 0.05） in the depressed rats; （2） Compared with the normal rats, the p-tau expression of CUMS group showed a obvious increase （P 〈 0.05） in rats submitted to CUMS, which had statistical significance. Conclusions These findings provide evidence that rats exposed to CUMS showed increased p-tau expression which suggests impairment of structural neuronal plasticity, providing insight into the link between depression and Alzheimer disease.