人参总皂甙对大鼠膈肌收缩能力的影响

目的: 探讨人参总皂甙(GS)对疲劳大鼠膈肌收缩能力的影响。方法: SD大鼠12只,随机分为两组。疲劳组6只,予低频电刺激(刺激参数:频率5Hz,电压20V,波宽2ms,串脉冲数5,串间隔330ms)10min诱发疲劳,静置30min;人参总皂甙(GS)组6只,予低频电刺激(参数如上述)10min诱发疲劳,应用人参总皂甙160mg/L灌流30min。分别测量其颤搐张力(Pt)、最大强直张力(P₀)、力-频率曲线、峰值收缩时间(CT)、半舒张时间(1/2RT)、最大收缩速率(+dT/dtmax)及最大舒张速率(-dT/dtmax)。结果: GS组大鼠膈肌P₀高于疲劳组(P<0.05);GS组大鼠膈肌1/2RT短于疲劳组(P<0.05);GS组大鼠膈肌+dT/dtmax及-dT/dtmax均高于疲劳组(P<0.05)。结论: 人参总皂甙能改善疲劳大鼠膈肌的收缩能力。

Influence of ginsenosides on contractility of fatigued diaphragm in rats

Objective:To study the effect of GS on fatigued diaphragm of rats.Methods:Twelve SD rats were divided into two groups at random.Fatigued group (n=6) were induced to fatigue by using a 10 min stimulation program consisting of 30 trains/min of five impulses each at 5 Hz(2 ms impulse duration at supramaximal voltage),and equilibrated for 30 min;GS group were induced to fatigue and perfused 160 mg/L GS solution for 30 min.In the end,Pt,P 0,CT,1/2RT,+dT/dtmax,-dT/dtmax and force-frequency relation were measured.Results:Compared with control group,P 0,+dT/dtmax,-dT/dtmax of GS group increased;CT,1/2RT of GS group was shorter than that of control group.Conclusions:GS can improve contractility of fatigued diaphragm.