| 溪黄草DNA 条形码鉴定研究 |
| |
| 引用本文： | 张慧晔,刘锋,王德勤,詹若挺,马新业,陈蔚文.溪黄草DNA 条形码鉴定研究[J].世界科学技术-中医药现代化,2014,16(7):1487-1490. |
| |
| 作者姓名： | 张慧晔 刘锋 王德勤 詹若挺 马新业 陈蔚文 |
| |
| 作者单位： | 广州白云山和记黄埔中药有限公司广州510515;广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室广州510006;广州白云山和记黄埔中药有限公司广州510515;广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室广州510006;广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室广州510006;广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室 广州 510006 |
| |
| 基金项目： | 广东省科技厅科技计划项目（2012A061600005）：广东省岭南药用植物资源保护与利用企业重点实验室（产学研）培育基地，负责人：王德勤；国家自然科学基金委青年基金项目（81102764）：基于改进Mauve 算法的贯众类药材DNA 条形码序列预测与验证，负责人：马新业；广东省科技厅博士启动项目（S2011040005237）：广东省水龙骨科药用植物资源DNA 条形码鉴定研究，负责人：马新业；广东省教育厅高等院校学科与专业建设专项（重点实验室滚动支持）（2013CXZDA011）：春砂仁等“十大广药”DNA 条形码标准化研究，负责人：陈蔚文。 |
| |
| 摘 要： | 目的：研究DNA条形码技术在溪黄草药材品种鉴定中的适用性。方法：选取rbcL、psbA -trnH、matK 和ITS2 等4 个条形码标记，对41 份溪黄草药材样品（含溪黄草Rabdosia serra、线纹香茶菜R. lo原phanthoides 和纤花香茶菜R. ophanthoides var. graciliflora）进行DNA 提取、PCR 扩增及双向测序，对测序结果进行质量检查、人工校对和序列拼接获取标准碱基序列信息，通过比较序列获得成功率以及物种鉴定力（TaxonGap 法），筛选适合溪黄草基原鉴定的DNA 条形码序列。结果：条形码序列获得成功率依次为rbcL（100%）、psbA -trnH（90.2%）、ITS2（87.8%）和matK（70.7%）；物种鉴定力方面，rbcL、psbA -trnH 和ITS2 等3条序列均可有效鉴定不同物种，但不适于分辨种下单位。结论：综合考察序列易得性和物种鉴定力，rbcL可作为溪黄草品种鉴定的首选条形码。
|
| 关 键 词： | 溪黄草 DNA 条形码 rbcL 鉴定 |
| 收稿时间： | 7/2/2014 12:00:00 AM |
| 修稿时间： | 2014/7/18 0:00:00 |
Differentiation on Original Plants of Xi-Huang-Cao through DNA Barcodes |
| |
| Zhang Huiye,Liu Feng,Wang Deqin,Zhan Ruoting,Ma Xinye and Chen Weiwen.Differentiation on Original Plants of Xi-Huang-Cao through DNA Barcodes[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2014,16(7):1487-1490. |
| |
| Authors: | Zhang Huiye Liu Feng Wang Deqin Zhan Ruoting Ma Xinye and Chen Weiwen |
| |
| Institution: | Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Company Ltd., Guangzhou 510515, China;Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Company Ltd., Guangzhou 510515, China;Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine,Guangzhou 510006, China |
| |
| Abstract: | This study was aimed to identify original plants of Xi-Huang-Cao (XHC) through DNA barcodes. The nucleotide sequence information of rbcL, psbA -trnH, matK and ITS2 regions were abstracted using standardized manners from 41 samples (including Rabdosia serra, R. lophanthoides and R. lophanthoides var. graciliflora). Sequencing efficiency of each marker was calculated. Species identification capability was tested on the basis of TaxonGap. The results showed that sequence success rates were 100% for rbcL, 90.2% for psbA -trnH, 87.8% for ITS2, and 70.7% for matK. Three markers (rbcL, psbA -trnH and ITS2) were competent for species discrimination (not for subspecies).It was concluded that rbcL can be the preferred barcode for XHC because of its convenience and efficacy. |
| |
| Keywords: | Xi-Huang-Cao DNA barcodes rbcL identification |
|
| 点击此处可从《世界科学技术-中医药现代化》浏览原始摘要信息 |
| 点击此处可从《世界科学技术-中医药现代化》下载免费的PDF全文 |
|
