| 鼠星形胶质细胞重编程为诱导多潜能干细胞的研究 |
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| 引用本文： | 许杰,闫秋月,唐洲平,湛彦强,吴军,李昌盛,李珍,肖连臣,张苏明.鼠星形胶质细胞重编程为诱导多潜能干细胞的研究[J].华中科技大学学报(医学版),2011,40(1):5-8. |
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| 作者姓名： | 许杰 闫秋月 唐洲平 湛彦强 吴军 李昌盛 李珍 肖连臣 张苏明 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目，武汉科技计划资助项目 |
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| 摘 要： | 目的研究星形胶质细胞重编程为诱导多潜能(iPS)干细胞的可行性,为进一步研究iPS细胞诱导技术奠定基础。方法用分别含Oct4,Sox2,Klf4和c-myc因子的4种逆转录病毒载体病毒颗粒感染星形胶质细胞,获得iPS细胞并进行鉴定。结果感染后第28天具有胚胎干细胞形态的克隆出现,诱导效率为(0.015±0.005)%,且碱性磷酸酶(AP)染色及SSEA-1和Oct4免疫荧光染色均显阳性。结论星形胶质细胞可以重编程为iPS细胞,进一步证明了iPS细胞诱导技术的普遍适用性。
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| 关 键 词： | 星形胶质细胞 诱导多潜能干细胞 重编程 |
Generation of Induced Pluripotent Stem Cells by Reprogramming of Mouse Astrocytes |
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| Xu Jie,Yan Qiuyue,Tang Zhouping et al.Generation of Induced Pluripotent Stem Cells by Reprogramming of Mouse Astrocytes[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(1):5-8. |
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| Authors: | Xu Jie Yan Qiuyue Tang Zhouping |
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| Institution: | Xu Jie,Yan Qiuyue,Tang Zhouping et al Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong Universityof Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the feasibility of reprogramming mouse astrocytes into induced pluripotent stem(iPS)cells.Methods Astrocytes were infected with retroviruses expressing Oct4,Sox2,Klf4,and c-Myc respectively.iPS cells were harvested and identified.Results The clones appeared on the day 28 after the infection,the reprogramming efficiency was(0.015±0.005)%,and the clones were positive for alkaline phosphatase activity,Oct4 and SSEA-1 staining.Conclusion Astrocytes could be reprogrammed into iPS cells,which demonstrated the universal applicability of reprogramming. |
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| Keywords: | astrocytes induced pluripotent stem cells reprogramming |
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